Logo-apb
Adv Pharm Bull. 2015;5(3): 329-334.
doi: 10.15171/apb.2015.046
PMID: 26504754
PMCID: PMC4616901
Scopus ID: 84983587921
  Abstract View: 1837
  PDF Download: 1056

Original Research

Cytoprotective Effects of Melatonin Against Amitriptyline-Induced Toxicity in Isolated Rat Hepatocytes

Shohreh Taziki 1,2, Mohammad Reza Sattari 3,4, Siavoush Dastamalchi 5, Mohammad Ali Eghbal 6,3*

1 Ischemic Disorders Research Center,Golestan University of Medical Sciences, Gorgan, Iran.
2 Students’ Research Committee, Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran.
3 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Neurosciences Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran.
5 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
6 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
*Corresponding Author: Department of Pharmacology and Toxicology, Faculty of pharmacy, Tabriz university of Medical Sciences, Tabriz, Iran Email m.a.eghbal@hotmail.com
*Corresponding Author: Email: m.a.eghbal@hotmail.com

Abstract

Purpose: Amitriptyline, one of the commonly used tricyclic antidepressants, caused rare but severe hepatotoxicity in patients who received it continuously. Previous findings showed that the intermediate metabolites of amitriptyline produced by CYP450 are involved in hepatic injury. Melatonin is an antiaging and antioxidant hormone synthesized from pineal gland. The aim of present study was to evaluate the protective role of melatonin in an in vitro model of isolated rat hepatocytes. Methods: Markers such as cell viability, reactive oxygen species formation, lipid peroxidation, mitochondrial membrane potential, and hepatocytes glutathione content were evaluated every 60 minutes for 180 minutes. Results: Present results indicated that administration of 1mM of melatonin effectively reduced the cell death, ROS formation and lipid peroxidation, mitochondrial membrane potential collapse, and reduced cellular glutathione content caused by amitriptyline. Conclusion: Our results indicated that melatonin is an effective antioxidant in preventing amitriptyline-induced hepatotoxicity. We recommend further in vivo animal and clinical trial studies on the hepatoprotective effects of melatonin in patients receiving amitriptyline.
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 1838

Your browser does not support the canvas element.


PDF Download: 1056

Your browser does not support the canvas element.

Submitted: 17 Aug 2014
Revision: 19 Nov 2014
ePublished: 19 Sep 2015
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)