Zead Helmi
1, Khaldun Mohammad Al Azzam
1,2*, Yuliya Tsymbalista
3, Refat Abo Ghazleh
2, Hassan Shaibah
2, Hassan Aboul-Enein
41 Faculty of Pharmacy, Isra University, 11622 Amman, Jordan.
2 Pharmacy program, Batterjee Medical College for Science and Technology (BMC), 21442 Jeddah, Kingdom of Saudi Arabia.
3 Department of pharmacognosy and botany, National Medical University is the name of O.O.Bogomolets, Ukraine.
4 Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Tahrir Street, Dokki, Cairo 12311, Egypt.
Abstract
Purpose: To investigate, for the first time, the chemical composition of
essential oil of the tubers and leaves of Jerusalem artichoke
(Helianthus tuberosus L.), a species of sunflower native
to eastern North America, growing in Ukraine.
Methods: A hydrodistillation apparatus was used for the extraction of
volatile components and then it was analysed by gas chromatography
equipped with a split-splitless injector (split ratio, 1:50) and flame
ionization detector (FID). The oil was analyzed under linear temperature
programming applied at 4°C/min from 50°C - 340°C. Temperatures of the
injector and FID detector were maintained at 280°C and 300°C,
respectively. The chemical analysis of the oil was carried out using gas
chromatography coupled to mass spectrometry (GC-MS), to determine the
chemical composition of the volatile fraction.
Results: The essential oils content ranged from 0.00019 to 0.03486 and
0.00011 to 0.00205 (g/100g), in leaves and tubers, respectively. The
qualitative and quantitative analysis led to the identification of 17
components in both species samples. The major component found in leaves
and tubers was (-)-β-bisabolene with 70.7% and 63.1%, respectively.
Conclusion:> Essential oil profile of Jerusalem artichoke species
showed significant differences between leaves and tubers species.
Additionally, the leaves of Jerusalem artichoke are a promising source
of natural β-bisabolene.