Adv Pharm Bull. 2014;4(6):521-526.
doi: 10.5681/apb.2014.077
PMID: 25671184
PMCID: PMC4312400
Scopus id: 84921975556
  Abstract View: 519
  PDF Download: 232

Original Research

Analysis of Essential Oil in Jerusalem Artichoke (Helianthus tuberosus L.) Leaves and Tubers by Gas Chromatography-Mass Spectrometry

Zead Helmi 1, Khaldun Mohammad Al Azzam 1,2 * , Yuliya Tsymbalista 3, Refat Abo Ghazleh 2, Hassan Shaibah 2, Hassan Aboul-Enein 4

1 Faculty of Pharmacy, Isra University, 11622 Amman, Jordan.
2 Pharmacy program, Batterjee Medical College for Science and Technology (BMC), 21442 Jeddah, Kingdom of Saudi Arabia.
3 Department of pharmacognosy and botany, National Medical University is the name of O.O.Bogomolets, Ukraine.
4 Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Tahrir Street, Dokki, Cairo 12311, Egypt.


Purpose: To investigate, for the first time, the chemical composition of essential oil of the tubers and leaves of Jerusalem artichoke (Helianthus tuberosus L.), a species of sunflower native to eastern North America, growing in Ukraine. Methods: A hydrodistillation apparatus was used for the extraction of volatile components and then it was analysed by gas chromatography equipped with a split-splitless injector (split ratio, 1:50) and flame ionization detector (FID). The oil was analyzed under linear temperature programming applied at 4°C/min from 50°C - 340°C. Temperatures of the injector and FID detector were maintained at 280°C and 300°C, respectively. The chemical analysis of the oil was carried out using gas chromatography coupled to mass spectrometry (GC-MS), to determine the chemical composition of the volatile fraction. Results: The essential oils content ranged from 0.00019 to 0.03486 and 0.00011 to 0.00205 (g/100g), in leaves and tubers, respectively. The qualitative and quantitative analysis led to the identification of 17 components in both species samples. The major component found in leaves and tubers was (-)-β-bisabolene with 70.7% and 63.1%, respectively. Conclusion:> Essential oil profile of Jerusalem artichoke species showed significant differences between leaves and tubers species. Additionally, the leaves of Jerusalem artichoke are a promising source of natural β-bisabolene.
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