Adv Pharm Bull. 2016;6(4):541-549.
doi: 10.15171/apb.2016.068
PMID: 28101461
PMCID: PMC5241412
Scopus id: 85011411608
  Abstract View: 383
  PDF Download: 258

Research Article

Acute and Subchronic Toxicity Study of the Median Septum of Juglans regia in Wistar Rats

Asma Ravanbakhsh 1, Majid Mahdavi 2 * , Ghader Jalilzade-Amin 2, Shahram Javadi 2, Masoud Maham 2, Daryosh Mohammadnejad 3, Mohammad Reza Rashidi 4 *

1 Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
3 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.


Purpose: Median septum of Juglans regia L. (Juglandaceae) with anti-diabetic effects has been used in Iranian traditional medicine. The present study estimates both oral acute and subchronic toxicities. Methods: In the oral acute toxicity study, female Wistar rats were treated with doses of 10, 100, 1000, 1600, 2900 and 5000 mg/ kg of the Juglans regia septum of methanol extract (JRSME), and were monitored for 14 days. In subchronic study, JRSME was administered by gavage at dose of 1000 mg/kg daily in Wistar rats for 28 days. Antioxidant status and biochemical examinations were fulfilled, and the vital organs were subjected to pathological analyses. Results: The extract did not produce any toxic signs or deaths; the medium lethal dose must be higher than 5000 mg/kg. In subchronic study, No significant morphological and histopathological changes were observed in the studied tissues. There was a significant increase in serum malondialdehyde (MDA) level in treated group compared to control after 4 weeks of JRSME intake. The treatment of rats resulted in a significant reduction of serum urea level (p<0.05), kidney’s xanthine dehydrogenase (XDH) activity (p<0.001) and elevation of aldehyde oxidase (AO) activity (p<0.05) in kidney. In the treated group, the mean diameter of glomerulus and proximal urine tube epithelium stature was slightly greater than control group. A significant increase in serum MDA level is subject for further studies. Conclusion: This study showed that the extract has no acute or subacute adverse effects with dose of 1000 mg/kg. The administration of JRSME may improve kidney structure and function and help in treatment of some chronic diseases.
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