Logo-apb
Adv Pharm Bull. 2017;7(4): 637-643.
doi: 10.15171/apb.2017.076
PMID: 29399554
PMCID: PMC5788219
Scopus ID: 85043370193
  Abstract View: 1572
  PDF Download: 1425

Research Article

siRNA-Mediated Silencing of CIP2A Enhances Docetaxel Activity Against PC-3 Prostate Cancer Cells

Saiedeh Razi Soofiyani 1,2, Akbar Mohammad Hoseini 1, Ali Mohammadi 1, Vahid Khaze Shahgoli 1, Behzad Baradaran 1*, Mohammad Saeid Hejazi 3,2*

1 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Department of Molecular Medicine, Faculty of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
*Corresponding Authors: Email: behzad_im@yahoo.com; Email: msaeidhejazi@yahoo.com

Abstract

Purpose: Cancerous inhibitor of protein phosphatase 2A (CIP2A) is an identified human oncoprotein which modulates malignant cell growth. It is overexpressed in human prostate cancer and in most of the human malignancies. The aim of this study was to investigate the effects of CIP2A silencing on the sensitivity of PC-3 prostate cancer cells to docetaxel chemotherapy. Methods: PC-3 cells were transfected using CIP2A siRNA. CIP2A mRNA and protein expression were assessed after CIP2A gene silencing using q-RT PCR and Western blotting. Proliferation and apoptosis were analyzed after treatment with docetaxol using MTT assay, DAPI staining, and flow cytometry, respectively. Results: Silencing of CIP2A enhanced the sensitivity of PC-3 cells to docetaxel by strengthening docetaxel induced cell growth inhibition and apoptosis against PC-3 cells. Conclusion: Silencing of CIP2A may potentiate the cytotoxic effects of docetaxel and this might be a promising therapeutic approach in prostate cancer treatment.
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 1573

Your browser does not support the canvas element.


PDF Download: 1425

Your browser does not support the canvas element.

Submitted: 10 Oct 2017
Revision: 23 Oct 2017
Accepted: 31 Oct 2017
ePublished: 31 Dec 2017
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)