Adv Pharm Bull. 2014;4(1):61-67.
doi: 10.5681/apb.2014.010
PMID: 24409411
PMCID: PMC3885371
  Abstract View: 275
  PDF Download: 145

Original Research

Analysis of Carotenoid Production by Halorubrum sp. TBZ126; an Extremely Halophilic Archeon from Urmia Lake

Davood Naziri 1,2, Masoud Hamidi 1,3, Salar Hassanzadeh 1, Vahideh Tarhriz 1, Bahram Maleki Zanjani 2, Hossein Nazemyieh 4, Mohamm Amin Hejazi 5, Mohammad Saeid Hejazi 1 *

1 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Department of Agricultural Biotechnology, Faculty of Agriculture, Zanjan University, Zanjan, Iran.
3 Students’ Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
5 Branch for Northwest & West Region, Agricultural Biotechnology Research Institute of Iran (ABRII), Tabriz, Iran.

Abstract

Purpose: Carotenoids are of great interest in many scientific disciplines because of their wide distribution, diverse functions and interesting properties. The present report describes a new natural source for carotenoid production. Methods: Halorubrum sp., TBZ126, an extremely halophilic archaeon, was isolated from Urmia Lack following culture of water sample on marine agar medium and incubation at 30 °C. Then single colonies were cultivated in broth media. After that the cells were collected and carotenoids were extracted with acetone-methanol (7:3 v/v). The identification of carotenoids was performed by UV-VIS spectroscopy and confirmed by thin layer chromatography (TLC) in the presence of antimony pentachloride (SbCl5). The production profile was analyzed using liquid-chromatography mass spectroscopy (LC-MS) techniques. Phenotypic characteristics of the isolate were carried out and the 16S rRNA gene was amplified using polymerase chain reaction (PCR). Results: LC-MS analytical results revealed that produced carotenoids are bacterioruberin, lycopene and β-carotene. Bacterioruberin was found to be the predominant produced carotenoid. 16S rRNA analysis showed that TBZ126 has 100% similarity with Halorubrum chaoviator Halo-G*T (AM048786). Conclusion: Halorubrum sp. TBZ126, isolated from Urmia Lake has high capacity in the production of carotenoids. This extremely halophilic archaeon could be considered as a prokaryotic candidate for carotenoid production source for future studies.
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