Venus Haghshenas, Shohreh Fakhari, Sako Mirzaie, Mohammadreza Rahmani, Fariba Farhadifar, Sara Peirzadeh, Ali Jalili
*1 Department of Biochemistry, Research and Science, Islamic Azad University, Sanandaj, Iran.
2 Kurdistan Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
3 Department of Biochemistry, Sanandaj Branch, Islamic Azad University, Iran.
4 Department of Gynecology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Abstract
Purpose: Accumulating evidence indicates that glycyrrhizin (GZ) and its
hydrolyzed metabolite 18-β glycyrrhetinic acid (GA) exhibit
anti-inflammatory and anticancer activities. The objective of this study
was to examine the in vitro cytotoxic activity of GA on human ovarian
cancer A2780 cells. Methods: A2780 cells were cultured in RPMI1640
containing 10% fetal bovine serum. Cells were treated with different
doses of GA and cell viability and proliferation were detected by dye
exclusion and
3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide
(XTT) assays. Apoptosis induction and expression of Fas and Fas ligand
(FasL) were analyzed by flow cytometry. Results: We observed that GA
decreases cell viability and suppressed cells proliferation in a
dose-dependent manner as detected by dye-exclusion and XTT assayes. In
addition, our flow cytometry data show that GA not only induces
apoptosis in A2780 cells but also upregulates both Fas and FasL on these
cells in a dose-dependent manner. Conclusion: we demonstrate that GA
causes cell death in A2780 cells by inducing apoptosis.