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Adv Pharm Bull. 2015;5(4): 587-591. doi: 10.15171/apb.2015.079
PMID: 26819933        PMCID: PMC4729348

Short Communication

Determination of Four Major Saponins in Skin and Endosperm of Seeds of Horse Chestnut (Aesculus Hippocastanum L.) Using High Performance Liquid Chromatography with Positive Confirmation by Thin Layer Chromatography

Zead Helmi Mahmoud Abudayeh 1 * , Khaldun Mohammad Al Azzam 2 * , Ahmad Naddaf 1, Uliana Vladimirovna Karpiuk 3, Viktoria Sergeevna Kislichenko 4

Cited by CrossRef: 12


1- Thomas P, Alhamd O, Iszkuło G, Dering M, Mukassabi T. Biological Flora of the British Isles: Aesculus hippocastanum. Journal of Ecology. 2019;107(2):992 [Crossref]
2- Kováč I, Melegová N, Čoma M, Takáč P, Kováčová K, Hollý M, Ďurkáč J, Urban L, Gurbáľová M, Švajdlenka E, Mojžišová G, Zajíček R, Szabo P, Mučaji P, Gál P. Aesculus hippocastanum L. Extract Does Not Induce Fibroblast to Myofibroblast Conversion but Increases Extracellular Matrix Production In Vitro Leading to Increased Wound Tensile Strength in Rats. Molecules. 2020;25(8):1917 [Crossref]
3- Góral I, Stochmal A, Wojciechowski K. Surface activity of the oat, horse chestnut, cowherb, soybean, quinoa and soapwort extracts – Is it only due to saponins?. Colloid and Interface Science Communications. 2021;42:100400 [Crossref]
4- Dias M, Albiston C, Añibarro-Ortega M, Ferreira I, Pinela J, Barros L. Sonoextraction of phenolic compounds and saponins from Aesculus hippocastanum seed kernels: Modeling and optimization. Industrial Crops and Products. 2022;185:115142 [Crossref]
5- Hien H, Hien P, Hung T. New HPLC pattern-oriented approach for quality control of enteric coated tablets containing aescin from Aesculus hippocastanum. Journal of Pharmaceutical and Biomedical Analysis. 2023;222:115106 [Crossref]
6- Góral I, Wojciechowski K. Surface activity and foaming properties of saponin-rich plants extracts. Advances in Colloid and Interface Science. 2020;279:102145 [Crossref]
7- Patil S, Adhyapak A, Shetti P, Gurao R. Development of a robust and reliable reverse-phase high-performance liquid chromatography (RP-HPLC) method using analytical quality by design principles for the accurate determination of esculin in its bulk form. Futur J Pharm Sci. 2023;9(1) [Crossref]
8- Owczarek-Januszkiewicz A, Kicel A, Olszewska M. Aesculus hippocastanum in the pharmaceutical industry and beyond – Phytochemistry, bioactivity, present application, and future perspectives. Industrial Crops and Products. 2023;193:116187 [Crossref]
9- Vanti G, Bani D, Salvatici M, Bergonzi M, Bilia A. Development and Percutaneous Permeation Study of Escinosomes, Escin-Based Nanovesicles Loaded with Berberine Chloride. Pharmaceutics. 2019;11(12):682 [Crossref]
10- Savarino P, Colson E, André J, Gerbaux P. Horse Chestnut Saponins–Escins, Isoescins, Transescins, and Desacylescins. Molecules. 2023;28(5):2087 [Crossref]
11- Colson E, Decroo C, Cooper-Shepherd D, Caulier G, Henoumont C, Laurent S, De Winter J, Flammang P, Palmer M, Claereboudt J, Gerbaux P. Discrimination of Regioisomeric and Stereoisomeric Saponins from Aesculus hippocastanum Seeds by Ion Mobility Mass Spectrometry. J Am Soc Mass Spectrom. 2019;30(11):2228 [Crossref]
12- Sikora F, Ochmian I, Sobolewska M. Quality and Technological Properties of Flour with the Addition of Aesculus Hippocastanum and Castanea Sativa . 2022;26(1):43 [Crossref]