ISSN: 2228-5881 eISSN: 2251-7308

Adv Pharm Bull. 2016;6(4): 495-507. doi: 10.15171/apb.2016.063

PMID: 28101456 PMCID: PMC5241407

Mini Review

Overview of Albumin and Its Purification Methods

Ramin Raoufinia ^1,2, Ali Mota ², Neda Keyhanvar ¹, Fatemeh Safari ¹, Sara Shamekhi ¹, Jalal Abdolalizadeh ^3,4 *

Cited by CrossRef: 78

1- Muraoka M, Justino A, Caixeta D, Queiroz J, Sabino-Silva R, Salmen Espindola F, Nagaraj R. Fructose and methylglyoxal-induced glycation alters structural and functional properties of salivary proteins, albumin and lysozyme. PLoS ONE. 2022;17(1):e0262369 [Crossref]

2- Wiedermann C. Phases of fluid management and the roles of human albumin solution in perioperative and critically ill patients. Current Medical Research and Opinion. 2020;36(12):1961 [Crossref]

3- Li J, Yu X, Martinez E, Zhu J, Wang T, Shi S, Shin S, Hassan S, Guo C. Emerging Biopolymer‐Based Bioadhesives. Macromolecular Bioscience. 2022;22(2) [Crossref]

4- Mehrizi T. Impact of Metallic, Quantum Dots and Carbon-Based Nanoparticles on Quality and Storage of Albumin Products for Clinical Use. NANO. 2021;16(14) [Crossref]

5- Wanat K, Brzezińska E. Statistical Methods in the Study of Protein Binding and Its Relationship to Drug Bioavailability in Breast Milk. Molecules. 2022;27(11):3441 [Crossref]

6- Mendes M, Rosa M, Coutinho J, Freire M, e Silva F. Improved accuracy in pentraxin-3 quantification assisted by aqueous biphasic systems as serum pretreatment strategies. International Journal of Biological Macromolecules. 2023;253:127540 [Crossref]

7- Phour A, Gaur V, Banerjee A, Bhattacharyya J. Recombinant protein polymers as carriers of chemotherapeutic agents. Advanced Drug Delivery Reviews. 2022;190:114544 [Crossref]

8- Tincu (Iurciuc) C, Andrițoiu C, Popa M, Ochiuz L. Recent Advancements and Strategies for Overcoming the Blood–Brain Barrier Using Albumin-Based Drug Delivery Systems to Treat Brain Cancer, with a Focus on Glioblastoma. Polymers. 2023;15(19):3969 [Crossref]

9- Chaurasiya A, Jaiswal M, Bayatigeri S, Kahar S, Tiwari S, Unnikrishnan A, Kulkarni M. Elevated Level of Glycated KQTALVELVK Peptide of Albumin Is Associated with the Risk of Diabetic Nephropathy. ACS Omega. 2023;8(23):20654 [Crossref]

10- POLTEP K, TESENA P, YINGCHUTRAKUL Y, TAYLOR J, WONGTAWAN T. Optimisation of a serum albumin removal protocol for use in a proteomic study to identify the protein biomarkers for silent gastric ulceration in horses. JES. 2018;29(3):53 [Crossref]

11- AGGARWAL S, DEVGUN M, NARANG R, LAL S, RANA A, GOYAL R. Thiazolopyrimidines: A Retrospective Study of Synthesis, Structure-Activity Relationship and Diverse Pharmacological Actions. Asian J Chem. 2022;34(3):519 [Crossref]

12- Nemergut M, Sedláková D, Fabriciová G, Belej D, Jancura D, Sedlák E. Explanation of inconsistencies in the determination of human serum albumin thermal stability. International Journal of Biological Macromolecules. 2023;232:123379 [Crossref]

13- Belinskaia D, Voronina P, Goncharov N. Integrative Role of Albumin: Evolutionary, Biochemical and Pathophysiological Aspects. J Evol Biochem Phys. 2021;57(6):1419 [Crossref]

14- Majid N, Siddiqi M, Alam A, Malik S, Ali W, Khan R. Cholic acid inhibits amyloid fibrillation: Interplay of protonation and deprotonation. International Journal of Biological Macromolecules. 2022;221:900 [Crossref]

15- Diebel L, Liberati D, Carge M. Effect of albumin solutions on endothelial oxidant injury: A microfluidic study. Surgery. 2023;173(3):876 [Crossref]

16- Edwards T, Koenig A, Thomas L, Sadik A, Edwards J. Purification of canine albumin by heat denaturation in a plasma bag. J Vet Emergen Crit Care. 2020;30(3):264 [Crossref]

17- Mishra V, Heath R. Structural and Biochemical Features of Human Serum Albumin Essential for Eukaryotic Cell Culture. IJMS. 2021;22(16):8411 [Crossref]

18- Hassanian M, Aryapour H, Goudarzi A, Javan M. Are zinc oxide nanoparticles safe? A structural study on human serum albumin using in vitro and in silico methods. Journal of Biomolecular Structure and Dynamics. 2021;39(1):330 [Crossref]

19- Abarova S, Stoitchkova K, Tzonev S, Argirova M, Yancheva D, Anastassova N, Tenchov B. Spectroscopic and thermodynamic characterization of the interaction of a new synthesized antitumor drug candidate 2H4MBBH with human serum albumin. PHAR. 2024;71:1 [Crossref]

20- Simon T, Schumann P, Bieri M, Schirner K, Widmer E. Hyperoncotic human albumin solutions for intravenous fluid therapy: Effectiveness of pathogen safety and purification methods, and clinical safety. Biosafety and Health. 2023;5(1):21 [Crossref]

21- Mangiacotte N, Prosperi-Porta G, Liu L, Dodd M, Sheardown H. Mucoadhesive Nanoparticles for Drug Delivery to the Anterior Eye. Nanomaterials. 2020;10(7):1400 [Crossref]

22- Gilg A, Harms M, Olari L, Urbanowitz A, Bonig H, Münch J. Absence of the CXCR4 antagonist EPI-X4 from pharmaceutical human serum albumin preparations. J Transl Med. 2021;19(1) [Crossref]

23- Kuten Pella O, Hornyák I, Horváthy D, Fodor E, Nehrer S, Lacza Z. Albumin as a Biomaterial and Therapeutic Agent in Regenerative Medicine. IJMS. 2022;23(18):10557 [Crossref]

24- Özdemir S. Inflammation: Complexity and significance of cellular and molecular responses. 2024;13(1):3 [Crossref]

25- Bae C, Ahn T. Albumin infusion ameliorates liver injury in streptozotocin-induced diabetic rats. Vet Med. 2022;67(5):245 [Crossref]

26- Shojai S, Haeri Rohani S, Moosavi-Movahedi A, Habibi-Rezaei M. Human serum albumin in neurodegeneration. 2022;33(7):803 [Crossref]

27- Jan K, Mohapatra S, Moon T. Alternative blood products in trauma. 2023;36(2):153 [Crossref]

28- Tanjung Y, Dewi M, Gatera V, Barliana M, Joni I, Chaerunisaa A. Factors Affecting the Synthesis of Bovine Serum Albumin Nanoparticles Using the Desolvation Method. NSA. 2024;Volume 17:21 [Crossref]

29- Nguyen M, Heo Y, Do B, Baek S, Kim C, Jang Y, Lee W, Choe H. Bacterial overexpression and purification of soluble recombinant human serum albumin using maltose-binding protein and protein disulphide isomerase. Protein Expression and Purification. 2020;167:105530 [Crossref]

30- Fu J, Gu M, Tang X, Zhang Q, Li P. Early evaluation of aflatoxin occurrence risk using an ultra-sensitive immunoassay for pre-aflatoxin protein biomarker detection. Food Control. 2024;160:110317 [Crossref]

31- Belinskaia D, Voronina P, Shmurak V, Jenkins R, Goncharov N. Serum Albumin in Health and Disease: Esterase, Antioxidant, Transporting and Signaling Properties. IJMS. 2021;22(19):10318 [Crossref]

32- Zhang K, Huang H, Hung H, Leng C, Wei S, Crisci R, Jiang S, Chen Z. Strong Hydration at the Poly(ethylene glycol) Brush/Albumin Solution Interface. Langmuir. 2020;36(8):2030 [Crossref]

33- Palmer J, Lord M, Pinyon J, Wise A, Lovell N, Carter P, Enke Y, Housley G, Green R. Comparing perilymph proteomes across species. The Laryngoscope. 2018;128(1) [Crossref]

34- De Angelis V, Breda A. Trends in Plasma Toll Fractionation for Self Sufficiency of Plasma-Derived Medicinal Products in Italy. Transfus Med Hemother. 2019;46(4):232 [Crossref]

35- Figueroa S, Araos P, Reyes J, Gravez B, Barrera-Chimal J, Amador C. Oxidized Albumin as a Mediator of Kidney Disease. Antioxidants. 2021;10(3):404 [Crossref]

36- Jagdish R, Maras J, Sarin S. Albumin in Advanced Liver Diseases: The Good and Bad of a Drug!. Hepatology. 2021;74(5):2848 [Crossref]

37- Turkina A, Mayevskaya M, Zharkova M, Ivashkin V. Structure and Functions of Human Serum Albumin in Normal Conditions and in Patients with Liver Cirrhosis. Rossijskij žurnal gastroènterologii, gepatologii, koloproktologii. 2022;32(4):7 [Crossref]

38- Long K, Rbaibi Y, Gliozzi M, Ren Q, Weisz O. Differential kidney proximal tubule cell responses to protein overload by albumin and its ligands. American Journal of Physiology-Renal Physiology. 2020;318(3):F851 [Crossref]

39- Visentini F, Perez A, Santiago L. Bioactive compounds: Application of albumin nanocarriers as delivery systems. Critical Reviews in Food Science and Nutrition. 2023;63(25):7238 [Crossref]

40- Mansur S, Othman M, Abidin M, Malek N, Ismail A, Kadir S, Goh P, Abdullah M, Asraf M. Dual-layer hollow fibre haemodialysis membrane for effective uremic toxins removal with minimal blood-bacteria contamination. Alexandria Engineering Journal. 2022;61(12):10139 [Crossref]

41- Khatoon B, Siddiquee M, Nadeem M, Tanweer S, Ahmed J, Patel R, Rizvi M, Rao G, Sharma K, Hoda N, Behera K. Synthesis, characterization, and anticancer activity of 4,6-dichloropyrimidine chalcone hybrids and their mechanistic interaction studies with HSA: In-silico and spectroscopic investigations. Journal of Molecular Structure. 2024;1303:137532 [Crossref]

42- Pires I, Govender K, Munoz C, Williams A, O'Boyle Q, Savla C, Cabrales P, Palmer A. Purification and analysis of a protein cocktail capable of scavenging cell‐free hemoglobin, heme, and iron. Transfusion. 2021;61(6):1894 [Crossref]

43- Siemiradzka W, Franczyk A, Bułaś L, Dolińska B. Somatotropin Penetration Testing from Formulations Applied Topically to the Skin. Applied Sciences. 2023;13(4):2588 [Crossref]

44- Gostomska-Pampuch K, Gamian A, Rawicz-Pruszyński K, Gęca K, Tkaczuk-Włach J, Jonik I, Ożga K, Staniszewska M. Proteins in human body fluids contain in vivo antigen analog of the melibiose-derived glycation product: MAGE. Sci Rep. 2022;12(1) [Crossref]

45- Abarca-Cabrera L, Xu L, Berensmeier S, Fraga-García P. Competition at the Bio-nano Interface: A Protein, a Polysaccharide, and a Fatty Acid Adsorb onto Magnetic Nanoparticles. ACS Appl Bio Mater. 2023;6(1):146 [Crossref]

46- Akbarzadehlaleh P, Kamani A, Shamsasenjan K. An efficient non-chromatographic method to separate human serum albumin from cryo-poor plasma. Separation Science and Technology. 2022;57(15):2491 [Crossref]

47- Jiang X, Mu H, Hsieh Y, Rao Q. Isolation and Characterization of Chicken Serum Albumin (Hen Egg Alpha-Livetin, Gal d 5). Foods. 2022;11(11):1637 [Crossref]

48- Francisco B, Sharma B, Russell H, Rosenfeldt L, Owens A, Flick M, Mullins E, Palumbo J. Pediatric patient with fibrinogen Villeurbanne II presenting with an unprovoked portal vein thrombosis. 2022;6(14):4297 [Crossref]

49- Hill D, Lang J, McCord J, Strynar M, Rosal C, Schmid J, Le T, Chernoff N. Variability of Microcystin-LR Standards Available from Seven Commercial Vendors. Toxins. 2022;14(10):705 [Crossref]

50- Roberts A, Whittall D, Breitling R, Takano E, Blaker J, Hay S, Scrutton N. Blood, sweat, and tears: extraterrestrial regolith biocomposites with in vivo binders. Materials Today Bio. 2021;12:100136 [Crossref]

51- Rufino A, Almeida M, Sharma M, Coutinho J, Freire M. Separation of Albumin from Bovine Serum Applying Ionic-Liquid-Based Aqueous Biphasic Systems. Applied Sciences. 2022;12(2):707 [Crossref]

52- Abu Elella M. Synthesis and Potential Applications of Modified Xanthan Gum. J Chem Eng Res Updates. 2021;8:73 [Crossref]

53- Starnes H, Jackson T, Rock K, Belcher S. Quantitative cross-species comparison of serum albumin binding of per- and polyfluoroalkyl substances from five structural classes. 2024; [Crossref]

54- Potrich C, Lunelli L, Bagolini A, Bellutti P, Pederzolli C, Verotti M, Belfiore N. Innovative Silicon Microgrippers for Biomedical Applications: Design, Mechanical Simulation and Evaluation of Protein Fouling. Actuators. 2018;7(2):12 [Crossref]

55- Sheikh-Jalali H, Mohseni-Shahri F, Moeinpour F. Evaluation of binding properties of bovine serum albumin and pyrimidine ligand: Spectroscopic and molecular docking approach. Journal of Molecular Structure. 2022;1252:132222 [Crossref]

56- Pratama J, Lestari W, Rofida A, Putri A, Widian R, Gunawan T, Hastuti D, Sulistiono D, Sari K. Novel polymer composite coated with ethylcellulose nanoparticle from waste paper as an alternative material to extracorporeal oxygenation membrane. J Polym Res. 2023;30(6) [Crossref]

57- Abu Elella M, Sabaa M, Hanna D, Abdel-Aziz M, Mohamed R. Antimicrobial pH-sensitive protein carrier based on modified xanthan gum. Journal of Drug Delivery Science and Technology. 2020;57:101673 [Crossref]

58- Hayat M, Bukhari S, Irfan M. Electrospinning of bovine serum albumin‐based nano‐fibers: From synthesis to medical prospects; Challenges and future directions. Biotechnology Journal. 2023;18(12) [Crossref]

59- Liu B, Li X, Zhang J, Li X, Yuan Y, Hou G, Zhang H, Zhang H, Li Y, Mezzenga R. Protein Nanotubes as Advanced Material Platforms and Delivery Systems. Advanced Materials. 2024;36(6) [Crossref]

60- Rios A, Ribeiro A, Rodrigues A, Ferreira A. Bovine serum albumin and myoglobin separation by size exclusion SMB. Journal of Chromatography A. 2020;1628:461431 [Crossref]

61- Hiller T, Berg J, Elomaa L, Röhrs V, Ullah I, Schaar K, Dietrich A, Al-Zeer M, Kurtz A, Hocke A, Hippenstiel S, Fechner H, Weinhart M, Kurreck J. Generation of a 3D Liver Model Comprising Human Extracellular Matrix in an Alginate/Gelatin-Based Bioink by Extrusion Bioprinting for Infection and Transduction Studies. IJMS. 2018;19(10):3129 [Crossref]

62- Shajib M, Futrega K, Franco R, McKenna E, Guillesser B, Klein T, Crawford R, Doran M. Method for manufacture and cryopreservation of cartilage microtissues. J Tissue Eng. 2023;14 [Crossref]

63- Maines E, Porwal M, Ellison C, Reineke T. Sustainable advances in SLA/DLP 3D printing materials and processes. Green Chem. 2021;23(18):6863 [Crossref]

64- Undiano E, Millán-Pacheco C, Ayala M, Monroy-Noyola A. Computational study to find the goat serum albumin (GSA) binding site as A-esterase. Chemico-Biological Interactions. 2023;381:110564 [Crossref]

65- Raoufinia R, Balkani S, Keyhanvar N, Mahdavipor B, Abdolalizadeh J. Human albumin purification: a modified and concise method. Journal of Immunoassay and Immunochemistry. 2018;39(6):687 [Crossref]

66- Chaudhari P, Wanjari D. A cross-sectional study on serum uric acid as an early marker in predicting the mortality and morbidity in patients with sepsis. F1000Res. 2024;13:182 [Crossref]

67- Muir V, Burdick J. Chemically Modified Biopolymers for the Formation of Biomedical Hydrogels. Chem Rev. 2021;121(18):10908 [Crossref]

68- Adamik K, Yozova I. Colloids Yes or No? - a “Gretchen Question” Answered. Front Vet Sci. 2021;8 [Crossref]

69- Zurita J, Motwani H, Ilag L, Souliotis V, Kyrtopoulos S, Nilsson U, Törnqvist M. Detection of Benzo[a]pyrene Diol Epoxide Adducts to Histidine and Lysine in Serum Albumin In Vivo by High-Resolution-Tandem Mass Spectrometry. Toxics. 2022;10(1):27 [Crossref]

70- Bhuyan N, Joardar A, Bag B, Chakraborty H, Mishra A. Exploring the inclusion complex formation of 3-acetylcoumarin with β-cyclodextrin and its delivery to a carrier protein: A spectroscopic and computational study. Journal of Molecular Liquids. 2021;344:117752 [Crossref]

71- Xu L, Bajorski P, Poligone B, Yue J. Lysophosphatidic acid down-regulates human RIPK4 mRNA in keratinocyte- derived cell lines. PLoS ONE. 2024;19(4):e0287444 [Crossref]

72- Li S, Li L, Chen Z, Xue G, Jiang L, Zheng K, Chen J, Li R, Yuan C, Huang M. A novel purification procedure for recombinant human serum albumin expressed in Pichia pastoris. Protein Expression and Purification. 2018;149:37 [Crossref]

73- Saravi B, Goebel U, Hassenzahl L, Jung C, David S, Feldheiser A, Stopfkuchen-Evans M, Wollborn J. Capillary leak and endothelial permeability in critically ill patients: a current overview. ICMx. 2023;11(1) [Crossref]

74- Schiefer E, Santos A, Muller M, Stinghen A, Negri L, Fabris J. Protein-Bound Uremic Toxins Quantification by a Colorimetric Sensor Based on the Oxidation of Silver Nanoparticles. IEEE Sensors J. 2021;21(20):22651 [Crossref]

75- Rios A, Ribeiro A, Rodrigues A, Ferreira A. Bovine Serum Albumin and Myoglobin Separation by Ion-Exchange SMB. Ind Eng Chem Res. 2023;62(35):13932 [Crossref]