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Adv Pharm Bull. 2020;10(4): 610-616. doi: 10.34172/apb.2020.073
PMID: 33072539        PMCID: PMC7539321

Research Article

Improving the Expression of Human Granulocyte Colony Stimulating Factor in Escherichia coli by Reducing the GC-content and Increasing mRNA Folding Free Energy at 5’-Terminal End

Kartika Sari Dewi 1 * ORCID, Asrul Muhamad Fuad 1

Cited by CrossRef: 3


1- Nuryana I, Laksmi F, Agustriana E, Dewi K, Andriani A, Thontowi A, Kusharyoto W, Lisdiyanti P. Expression of Codon-Optimized Gene Encoding Murine Moloney Leukemia Virus Reverse Transcriptase in Escherichia coli. Protein J. 2022;41(4-5):515 [Crossref]
2- Song Y, Wang Y, Yan S, Nakamura K, Kikukawa T, Ayabe T, Aizawa T. Efficient recombinant production of mouse-derived cryptdin family peptides by a novel facilitation strategy for inclusion body formation. Microb Cell Fact. 2023;22(1) [Crossref]
3- Paremskaia A, Kogan A, Murashkina A, Naumova D, Satish A, Abramov I, Feoktistova S, Mityaeva O, Deviatkin A, Volchkov P. Codon-optimization in gene therapy: promises, prospects and challenges. Front Bioeng Biotechnol. 2024;12 [Crossref]
4- Dewi K, Wahyuni F, Salsabila S, Salsabila A, Yanthi N, Fuad A. Expression comparison between two genes encoding CSF3 recombinant proteins having different codon composition at N-terminal in Escherichia coli . IOP Conf Ser: Earth Environ Sci. 2021;948(1):012081 [Crossref]
5- Zabolotskii A, Riabkova N. A new look at the fluorescent protein‐based approach for identifying optimal coding sequence for recombinant protein expression in E. coli. Biotechnology Journal. 2024;19(4) [Crossref]