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Adv Pharm Bull. 2017;7(3): 427-432.
doi: 10.15171/apb.2017.051
PMID: 29071225
PMCID: PMC5651064
Scopus ID: 85030116479
  Abstract View: 2068
  PDF Download: 2255

Research Article

Nanoparticles of Chitosan Loaded Ciprofloxacin: Fabrication and Antimicrobial Activity

Zahra Sobhani 1,2, Soliman Mohammadi Samani 3*, Hashem Montaseri 1, Elham Khezri 1

1 Department of quality control, Faculty of pharmacy, Shiraz University of Medical Science, Shiraz, Iran.
2 Center for nanotechnology in drug delivery, Faculty of pharmacy, Shiraz University of Medical Science, Shiraz, Iran.
3 Department of pharmaceutics, Faculty of pharmacy, Shiraz University of Medical Science, Shiraz, Iran.
*Corresponding Author: Email: smsamani@sums.ac.ir

Abstract

Purpose: Chitosan is a natural mucoadhesive polymer with antibacterial activity. In the present study, chitosan (CS) nanoparticles were investigated as a vehicle for delivery of antibiotic, ciprofloxacin hydrochloride. Methods: Ionotropic gelation method was used for preparation chitosan nanoparticles. The effects of various factors including concentration of CS, concentration of tripolyphosphate (TPP), and homogenization rate on the size of nanoparticles were studied. The effects of various mass ratios of CS to ciprofloxacin hydrochloride on the encapsulation efficiency of nanoparticles were assessed. Results: The particles prepared under optimal condition of 0.45% CS concentration, 0.45% TPP concentration and homogenizer rate at 6000 rpm, had 72 nm diameter. In these particles with 1:0.5 mass ratio of CS to ciprofloxacin hydrochloride, the encapsulation efficiency was 23%. The antibacterial activity of chitosan nanoparticles and ciprofloxacin-loaded nanoparticles against E.coli and S.aureus was evaluated by calculation of minimum inhibitory concentration (MIC). Results showed that MIC of ciprofloxacin loaded chitosan nanoparticles was 50% lower than that of ciprofloxacin hydrochloride alone in both of microorganism species. Nanoparticles without drug exhibited antibacterial activity at higher concentrations and MIC of them against E.coli and S.aureus was 177 and 277 µg/ml, respectively. Conclusion: Therefore chitosan nanoparticles could be applied as carrier for decreasing the dose of antibacterial agents in the infections.
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Submitted: 28 Jan 2017
Revision: 06 Aug 2017
Accepted: 15 Aug 2017
ePublished: 25 Sep 2017
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