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Adv Pharm Bull. 2017;7(2): 269-274.
doi: 10.15171/apb.2017.032
PMID: 28761829
PMCID: PMC5527241
Scopus ID: 85021420852
  Abstract View: 1856
  PDF Download: 1070

Research Article

Effects of Pomegranate (Punica Granatum L.) Seed and Peel Methanolic Extracts on Oxidative Stress and Lipid Profile Changes Induced by Methotrexate in Rats

Farideh Doostan 1, Roxana Vafafar 2, Parvin Zakeri-Milani 3, Aliasghar Pouri 3, Rogayeh Amini Afshar 4, Mehran Mesgari Abbasi 5*

1 Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran.
2 Department of Biology, Faculty of Science, Islamic Azad University, Ahar Branch, Ahar, Iran.
3 Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Faculty of Sciences, Urmia University, Urmia, Iran.
5 Student Research Committee, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
*Corresponding Author: Email: mesgarim@tbzmed.ac.ir

Abstract

Purpose: Methotrexate (MTX) is prescribed in many diseases and can result in oxidative stress (OS) followed by injuries in some tissues. Antioxidants administration are effective in reducing OS. Pomegranate exhibits high anti-oxidant capacities. This study investigated whether pomegranate seed and peel methanolic extracts (PSE and PPE) could protect against MTX-induced OS and lipid profile changes in rats. Methods: Forty-eight rats were randomly divided into 6 groups: control group (normal salin), PSE group (500 mg/kg, orally), PPE group (500 mg/kg, orally), MTX group (10 mg/kg, IM), MTX and PSE group, and MTX and PPE group. Blood samples were taken for analysis in the end of the procedure. Results: The findings showed a significant reduction in Glutathione peroxidase (GPx) and Superoxide dismutase (SOD), and an enhancement in malondialdehyde (MDA) values after MTX treatment (p < 0.05). SOD and GPx levels reached the levels of the control group in MTX+SPE and MTX+PPE groups. No significant differences were observed in catalase (CAT) and total antioxidant capacity (TAC) levels between groups. The results showed a significant decrease in total cholesterol (TC), low density lipoprotein (LDL), and high density lipoprotein (HDL) in the MTX treated group (p < 0.01). The values of TC, HDL, and LDL became elevated to the normal control levels in the MTX+PSE and MTX+PPE treated groups. Conclusion: The results showed the OS induced by MTX and the protective effects of PSE and PPE against MTX-induced serum oxidative stress and lipid profile changes in rats.
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Submitted: 02 Oct 2016
Revision: 28 May 2017
Accepted: 30 May 2017
ePublished: 30 Jun 2017
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