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Research Article

Preparation, Statistical Optimization and Characterization of Propolis-Loaded Solid Lipid Nanoparticles Using Box-Behnken Design

Sahar Taherzadeh 1 ORCID logo, Atefeh Naeimifar 2 ORCID logo, Ehsan Mehrabani Yeganeh 1, Zahra Esmaili 2, Reza Mahjoub 1 ORCID logo, Hamid Akbari Javar 2* ORCID logo

1 Department of Pharmaceutics, Faculty of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.
2 Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

Purpose: Propolis is a resinous material obtained by honeybees with many biological and pharmacological properties which can be used for treatment of various diseases. Current study aims to formulate and characterize propolis-loaded solid lipid nanoparticles (SLNs) carrier system.
Methods: The prepared SLNs, composed of glyceryl monostearate (GMS), soy lecithin, tween 80 and polyethylene glycol 400 (PEG 400), were fabricated employing solvent emulsification-evaporation technique. In addition, the impact of several variables including concentration ratios of GMS/soy lecithin and PEG 400/tween 80 along with emulsification time were evaluated on the size, polydispersity index (PDI) and zeta potential of particles. SLN formulations were optimized using Box-Behnken design. The particles were freeze dried and morphologically studied by scanning electron microscopy (SEM). The in-vitro release profile of propolis entrapped in the optimized nanoparticles was investigated.
Results: The mean particle size, PDI, zeta potential, entrapment efficiency (EE) and loading efficiency (LE) of optimized propolis-loaded SLNs were found to be 122.6±22.36 nm, 0.28±0.06, -26.18±3.3 mV, 73.57±0.86% and 3.29±0.27%, respectively. SEM images exhibited nanoparticles to be non-aggregated and in spherical shape. The in-vitro release study showed prolonged release of propolis from nanoparticles.
Conclusion: The results implied that the proposed way of SLN preparation could be considered as a proper method for production of propolise loaded colloidal carrier system.
Keywords: Propolis, Solid Lipid Nanoparticles (SLN), Drug delivery, Solvent emulsification-evaporation method, Box-Behnken design
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Submitted: 29 Dec 2019
Revision: 23 Apr 2020
Accepted: 19 Jun 2020
ePublished: 20 Jun 2020
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