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  Abstract View: 46

Research Article

Evaluating the Susceptibility Pattern of Caspofungin Coated Gold-Nanoparticles against Clinically Important Candida Species

Zahra Salehi ORCID logo, Azam Fattahi* ORCID logo, Ensieh lotfali, Abdolhassan Kazemi, Ali Shakeri-Zadeh, Saman Ahmad Nasrollahi

Abstract

Abstract Purpose: The authors hypothesized that caspofungin-coated gold-nanoparticles (AuNPs) may offer the right platform to induce sensitivity in resistant isolates. Methods: A total of 58 archived Candida species were enrolled in the study. The identification of Candida spp. was performed by polymerase chain reaction-restriction fragment length polymorphism and HWP1 gene amplification approaches. The conjugated CAS- AuNPs were synthesized and characterized using transmission electron microscopy and zeta-sizer to determine the morphology, size, and charge of CAS-AuNPs. The efficacy of CAS, CAS- AuNPs conjugate, and gold nanoparticles against Candida spp. was assessed using the Clinical and Laboratory Standards Institute M60. The interaction of CAS-AuNPs with Candida element was evaluated via scanning electron microscopy. Results: The synthesized CAS-AuNPs were observed in spherical shape with the average hydrodynamic size of the nanoparticles ranging from 7 to 15 nm. The Zeta potential of CAS-AuNPs was obtained as - 22.4 mV. Statistical analyses showed that CAS- AuNPs could significantly reduce the MIC against C. albicans (P = 0.0005) and non- albicans Candida (NAC) (P < 0.0001). Among all isolates, an MIC of ≥ 4 µg/ml for CAS was observed except for C. glabrata. SEM analysis confirmed the effects of AuNPs on the membrane and cell wall structure of C. globrata exposure to CAS-AuNPs through the formation of pores on the cell wall and finally cell death. Conclusions: CAS-AuNPs conjugates showed significant antifungal effects on Candida spp. through degradation of the membrane and cell wall integrity. Overall, the use of encapsulation of antifungal drugs in combination with NPs leads to diminished side effects and enhances the effectiveness of the drugs.
Keywords: Candida albicans, non- albicans Candida, CAS- AuNPs conjugated, Caspofungin
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Submitted: 28 Feb 2020
Revision: 16 Oct 2020
Accepted: 17 Oct 2020
ePublished: 18 Oct 2020
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