Logo-apb
  Abstract View: 73

Research Article

Studying the Effect of Amino Acid Substitutions in the M2 Ion Channel of the Influenza Virus on the Antiviral Activity of the Aminoadamantane Derivative in Vitro and in Silico

Timur Mansurovich Garaev* ORCID logo, Artyom Irorevich Odnovorov ORCID logo, Alexander Aleksandrovich Lashkov ORCID logo, Tatiana Vladimirovna Grebennikova ORCID logo, Marina Pavlovna Finogenova ORCID logo, Galina Kadymovna Sadykova ORCID logo, Alexei Gennadievich Prilipov ORCID logo, Tatiana Anatol'evna Timofeeva, Sergey Vadimovich Rubinsky, Svetlana Nikolaevna Norkina, Marina M. Zhuravleva

Abstract

Objective: The aminoadamantane derivative of L-histidyl-1-adamantayl ethylamine hydrochloride (HCl*H-His-Rim) has showed a high inhibition level against influenza A virus strains in vitro. The aim of this work is to search and establish evidence of the direct effect of the drug on influenza A virus proton channel M2. Methods: The compound HCl*H-His-Rim was obtained by classical peptide synthesis methods. Influenza A virus mutants of A/PuertoRico/8/34(H1N1) strain were obtained by reverse genetics methods. The mutant samples of the virus were cultured on chicken embryos with a virus titer in the hemagglutination test. ELISA was carried out on Madin-Darby Canine Kidney (MDCK) monolayer cells when multiplying the virus 10-4-10-6. The binding stability of HCl*H-His-Rim was compared to those of M2 (S31N) and M2 (S31N_A30T) channels by molecular dynamic (MD) modeling. The calculation was performed taking into account the interaction with the model lipid bilayer (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)) in the presence of water molecules in accordance with the three-center model. Results: It was found that HCl*H-His-Rim is a direct action drug against influenza A. The most likely conformation of drug binding to target protein has been shown. It has been found that the A30T mutation reduces the binding energy of the drug, and the results obtained in vitro have confirmed the data calculated in silico. Conclusion: The mechanism of action of HCl*H-His-Rim is directly related to the suppression of the function of the proton channel M2 of influenza A virus.

Keywords: antiviral, drug resistance, Influenza virus, M2 proton channel, molecular docking, reverse genetics
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 73

Your browser does not support the canvas element.


PDF Download: 0

Your browser does not support the canvas element.

Submitted: 05 Mar 2020
Revision: 03 Jul 2020
Accepted: 15 Jul 2020
ePublished: 15 Jul 2020
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)