Abstract View: 258

Research Article

Experimental Design Supported Liposomal Aztreonam Delivery: In Vitro Studies

Sayani Bhattacharyya 1* ORCID logo, Preethi Sudheer 1, Kuntal Das 1 ORCID logo, Subhabrata Ray 2 ORCID logo

1 Krupanidhi College of Pharmacy, Bengaluru, Karnataka 560035, India.
2 Dr. BC Roy College of Pharmacy, Durgapur, West Bengal 713206, India.


Purpose: The present study focuses on a systemic approach to develop liposomal aztreonam as a promising dosage form for inhalation therapy in the treatment of pneumonia and explores the in-vitro antimicrobial and cell uptake efficacy.
Methods: Liposomes were prepared by ethanol injection method using the lipids- soya phosphatidylcholine and cholesterol. A central composite design was employed to optimize the lipid composition to evaluate the effect on vesicle size, zeta potential and entrapment efficiency of the formulation. A numerical and graphical optimization was carried out to predict the optimized blend. The optimized formulation was characterized for vesicle size, surface charge, encapsulation, surface morphology, DSC, PXRD, TGA, in-vitro diffusion, accelerated stability studies, antimicrobial studies on Pseudomonas aeruginosa NCIM 2200 and in-vitro cell uptake studies.
Results: The optimized formulation was found to have a particle size of 144nm, a surface charge of -35 mV, with satisfactory drug entrapment. The surface morphology study proved the formation of nanosized vesicles. The drug release from liposomal matrix was biphasic in nature. The solid-state study revealed the reason for good encapsulation of drug. The moisture retention capacity was found to be minimum. The anti-microbial study revealed the potential antibacterial activity of the optimized formulation over the pure drug. The formulation was found to be safe on the epithelial cells and showed a marked increase in cellular uptake of aztreonam in a lipid carrier.
Conclusion: It can be concluded that the optimized liposomal aztreonam could be considered as a promising approach for the delivery of aztreonam through inhalation.
Keywords: Aztreonam, Liposomes, Statistical optimization, Antimicrobial study, Cell uptake study
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Abstract View: 258

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Submitted: 16 Jul 2020
Revision: 25 Aug 2020
Accepted: 15 Sep 2020
ePublished: 15 Sep 2020
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