Logo-apb
Adv Pharm Bull. 2023;13(3): 611-620.
doi: 10.34172/apb.2023.057
PMID: 37646060
PMCID: PMC10460813
  Abstract View: 429
  PDF Download: 212
  Full Text View: 61

Research Article

Enhanced Anti-cancer Potency Using a Combination of Oleanolic Acid and Maslinic Acid to Control Treatment Resistance in Breast Cancer

Cigir Biray Avcı 1* ORCID logo, Fatma Sogutlu 1, Neslihan Pinar Ozates 1, Behrouz Shademan 1 ORCID logo, Cumhur Gunduz 1

1 Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey.
*Corresponding Author: Cigir Biray Avci, Email: cigir.biray@ege.edu.tr

Abstract

Purpose: The phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin (PI3K/AKT/ mTOR) pathway is a complex intracellular metabolic pathway that leads to cell growth and tumor proliferation and plays a key role in drug resistance in breast cancer. Therefore, the anti-cancer effects of oleanolic acid (OA), maslinic acid (MA), and their combination were investigated to improve the performance of the treatment strategy.

Methods: We investigated the effect of OA and MA on cell viability using the WST-1 method. The synergistic effect of the combination was analyzed by isobologram analysis. In addition, the effects of the two compounds, individually and in combination, on apoptosis, autophagy, and the cell cycle were investigated in MCF7 cells. In addition, changes in the expression of PI3K/AKT/mTOR genes involved in apoptosis, cell cycle and metabolism were determined by quantitative RT-PCR.

Results: MA, OA, and a combination of both caused G0/G1 arrest. Apoptosis also increased in all treated groups. The autophagosomal LC3-II formation was induced 1.74-fold in the MA-treated group and 3.25-fold in the MA-OA-treated group. The combination treatment resulted in increased expression of genes such as GSK3B, PTEN, CDKN1B and FOXO3 and decreased expression of IGF1, PRKCB and AKT3 genes.

Conclusion: The results showed that the combination of these two substances showed the highest synergistic effect at the lowest dose and using MA-OA caused cancer cells to undergo apoptosis. The use of combination drugs may reduce the resistance of cancer cells to treatment.

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 430

Your browser does not support the canvas element.


PDF Download: 212

Your browser does not support the canvas element.


Full Text View: 61

Your browser does not support the canvas element.

Submitted: 26 Feb 2022
Revision: 11 Jul 2022
Accepted: 09 Sep 2022
ePublished: 18 Sep 2022
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)