Nanostructured Lipid Carriers: A Groundbreaking Approach for Transdermal Drug Delivery

Nanostructured lipid carriers (NLCs) are novel pharmaceutical formulations which are composed of physiological and biocompatible lipids, surfactants and co-surfactants. Over time, as a second generation lipid nanocarrier NLC has emerged as an alternative to first generation nanoparticles. This review article highlights the structure, composition, various formulation methodologies, and characterization of NLCs which are prerequisites in formulating a stable drug delivery system. NLCs hold an eminent potential in pharmaceuticals and cosmetics market because of extensive beneficial effects like skin hydration, occlusion, enhanced bioavailability, and skin targeting. This article aims to evoke an interest in the current state of art NLC by discussing their promising assistance in topical drug delivery system. The key attributes of NLC that make them a promising drug delivery system are ease of preparation, biocompatibility, the feasibility of scale up, non-toxicity, improved drug loading, and stability.


Introduction
. Advantages and disadvantages of Nanostructured Lipid Carriers [5][6][7] Advantages Disadvantages More loading capacity for some drugs Cytotoxic effects related to the nature of lipid matrix and concentration Less water in the dispersion Irritation and sensitizing action of surfactants Prevent or minimize the drug expulsion during storage Application and efficiency in case of protein and peptide drugs and gene delivery systems still need to be exploited Degree of crystallization of various lipids employed also affect drug entrapment and loading, size and charge, and efficacy. 12 On account of higher viscosity of dispersed phase, because of higher melting lipids, the average particle size of nano dispersion increases. Shape of lipid crystals, lipid hydrophilicity, variation in composition are additional lipid related parameters that may influence quality of NLC. It has been found that a 5-10% hike in lipid amount leads to larger particle size. 13

Surfactants
The type and concentrations of surfactant exert influence on quality and efficacy of NLC. It has been found that toxicity, physical stability and crystallinity of NLC are greatly influenced by choice of surfactant. 14 Surfactant systems also have an impact on extent of drug dissolution and drug permeability. Surfactants are chosen based on of route of administration, hydrophilic-lipophilic balance (HLB) value, effect on particle size and lipid modification. Surface active agents (emulsifiers) are adsorbed on the interface where they reduce the tension between lipid and aqueous phases because of their amphipathic nature. 5 During the formulation of NLC, crystallization of colloid particles goes along with solidification, but the surface area of particle increase remarkably during crystallization so that the whole system become unstable. Hence, surfactant is a requisite to improve interface quality of nanoparticles to attain stability. 15 Modifying the surfactant system compositions may govern the miscibility of chemical components in NLCs, and hence the stability. 14 Required HLB (rHLB) plays an important role while selecting suitable type and amount of surfactant for NLC formulation. 16 rHLB of lipids and lipid matrix is determined to calculate the amount of emulsifiers to be added in formulation. The rHLB value for lipid is the HLB value of emulsifier which is necessary for appropriate emulsification i.e. reduction of interfacial tension between oil and water phase. This also assists in achieving a stable nano system and small particle size of NLCs. 17,18 By determining rHLB a right combination of emulsifiers with least concentration can be employed for formulation. rHLB for lipids (solid and liquid) and lipid matrix is calculated experimentally by dispersing in blends of surfactant with different HLB values. The mixture is put through high pressure homogenization and analyzed for least particle size. 16,18,19 Other ingredients Organic salts and ionic polymers may be employed as counter-ions in formulation of nano structure carriers to overcome the challenge of encapsulating water soluble drug molecules. Surface-modifiers are another category of excipients used in formulation of NLC to minimize their phagocytic uptake by the macrophages in reticuloendothelial system (RES). Lipid particles are coated with hydrophilic polymers like PEG, poloxamines or poloxamers to increase the residence time of drug molecules in systemic circulation. Surface modification may offer other advantages like enhanced physical stability and biocompatibility, drug targeting, increased transport across epithelium. 10,20 Methods of preparing NLC High-pressure homogenization technique This technique is powerful and reliable for the commercialscale production of NLCs. High pressure used in homogenization technique makes it possible to avoid use of organic solvents in preparations and render them eco friendly. Additionally high-pressure homogenization is easy to scale up and an attractive technique being used in the manufacturing of pharmaceuticals and cosmetics for topical application. 21 Hot homogenisation is performed at elevated temperature and cold homogenization is done below room temperature. Active ingredient is dissolved or dispersed in the molten lipid before to the high pressure homogenization, in both approaches. High pressure (100-2000 bar) moves the fluid in the narrow gap in homogenizer.

Hot homogenization
In this approach homogenization is conducted at elevated temperature. The solid lipids are melted at a temperature above 5-10°C above their melting point. A dispersion is obtained by adding liquid lipid and drug to be encapsulated. The mixture is dispersed in aqueous

Solvent-emulsification evaporation method
In this method, the lipids (solid lipid + liquid lipid) along with drug are dissolved in a water immiscible organic solvent (cyclohexane, chloroform). 25 The obtained mixture is dispersed into aqueous solution of emulsifiers producing an o/w emulsion. Evaporation under reduced pressure is employed to remove solvent from the emulsion. Evaporation leads to the dispersion of nanoparticles in the aqueous phase (by lipid precipitation in the aqueous medium). This method avoids any thermal stress, but usage of organic solvent is a disadvantage. Particle size can vary from 30-100 nm according to the solid lipid and surfactant. 6,24

Solvent-emulsification diffusion method
In this technique, solvent and water are mutually saturated to maintain initial thermodynamic equilibrium. Afterwards, the lipids and drug is dissolved in the watersaturated solvent. Solvent containing drug and lipids are emulsified in a solvent-saturated aqueous emulsifier solution by a homogenizer to form an o/w emulsion. The lipid nanoparticles precipitate after dilution with excess water (ratio: 1:5-1:10) due to diffusion of the organic solvent from the emulsion droplets to the continuous phase. The solvent can be removed by ultrafiltration or lyophilisation. Solvent diffusion is more innovative and most of the solvent employed show a better safety profile compared to volatile solvents. 25

Microemulsion method
In this approach, the solid lipid is melted, followed by addition of liquid lipid and solubilization of drug in the subsequent mixture. Separately, a mixture of emulsifier, co-emulsifier and water is heated at same temperature. Both the lipid and the aqueous phase are mixed in appropriate ratios and gently stirred to produce thermodynamically stable oil in water hot microemulsion. The hot microemulsion is quickly dispersed into an excess of chilled water (0-4°C) with vigorous stirring. The dilution causes the breakdown of microemulsion into a nanoemulsion with ultrafine particles. The ratio of the hot microemulsion to cold water usually lies in the range of 1:10 to 1:50. As the microemulsion is diluted by cold water, the internal lipid droplets recrystallize to form nanosize carriers. The size of the nanoparticles depends on the droplet size of microemulsion and temperature difference between microemulsion and ice water. Rapid cooling and hence solidification can prevent the aggregation of particles and lead to production of smaller particles. 26 NLC dispersions formed by this method contained large quantity of particles in the micron range; therefore, in this condition, the time of stirring, percentage of lipids, and amount of drug were optimized in order to obtain the appropriate size and higher entrapment efficiency. The method does not require any special equipment or energy for production of NLC; hence it is simple to commercially scale up the technique. 22,27,28

Double emulsion technique
This method is mainly used for the production of lipid nanoparticles loaded with hydrophilic drugs. This technique overcomes the problem of escapism of water soluble moiety in aqueous phase from oily phase as investigated in microemulsion method. 29 In this method, drug is firstly dissolved in aqueous solvent (inner aqueous phase) and then is dispersed in lipid phase (Molten solid lipid + liquid lipid+ lipophilic surfactant+ lipophilic active moiety) to produce primary emulsion (w/o). Both lipid and the aqueous phase are maintained at same temperature. Stabilizer prevents loss of drug to the external phase during solvent evaporation. Afterwards, primary emulsion is dispersed into a large volume of surfactant aqueous solution followed by sonication to form a double emulsion (w/o/w). The lipid nanoparticles are then purified by ultrafiltration or solvent evaporation. 24,30 Solvent Injection technique It is a viable new technique to manufacture lipid nanoparticles. In this technique, lipids are solubilized in water-miscible solvent (e.g., acetone, methanol, ethanol, isopropyl alcohol) or water-soluble solvent mixture and then rapidly injected into aqueous surfactant solution under continuous stirring. Resultant dispersion is filtered in order to eliminate excess lipid. 31 The technique relies on rapid diffusion of the solvent over the solvent-lipid interfaced with the aqueous phase. 32 The particle size of nanocarriers depends on diffusion rate of the organic solvent through the lipid-solvent interface. This method offers the advantage of easy handling, efficiency, versatility, no employment of technical equipment (e.g., high-pressure homogenizer) and use of approved organic solvents. 31

High shear homogenization and ultrasonication
One of the methods for the production of NLCs is high shear homogenization or ultrasonification. These dispersing techniques employ devices to prepare nanocarriers. Solid and liquid lipid is melted and dispersed in an aqueous surfactant solution under high shear homogenization or ultrasonication resulting in formation of nanodispersion. 33,34 Intense shear forces necessary for the nano-emulsification are generated by ultrasonic cavitation which produces violently and asymmetrically imploding vacuum bubbles and break up particles down to the nanometer scale. 35 Probe-type ultrasonication produce desired effects like homogenization, dispersion, deagglomeration, milling and emulsification. 36 The type and concentration of lipid and surfactant, their ratio, time of sonication or agitation, speed are some of the parameters to be optimized to obtain a reproducible method resulting small size nanocarriers. Low dispersion quality is a disadvantage of high shear homogenization and ultrasonication. Dispersion quality of the lipid nanoparticles produced by these techniques is often affected by the presence of microparticles leading to physical instability upon storage. 37 Metal contaminations from the equipment is the other important problem associated with ultrasonication. 35

Phase inversion technique
It is a novel, cost effective and solvent-free approach for the formulation of lipid nanocarriers that involves the phase inversion from o/w to w/o emulsion. It involve two steps.
Step 1 involves mixing of all the ingredients (lipid, surfactant and water) in optimized proportions. The mixture is stirred and temperature is increased at a rate of 4°C to reach up to 85°C from room temperature. Three temperature cycles (85-60-85-60-85°C) are applied to the system to reach phase inversion zone.
Step 2 results an irreversible shock introduced to break the system, due to dilution with cold water (0 o C). This fast addition of cold water causes formation of nanocapsules. Application of a slow magnetic stirring for 5 minutes avoids particle aggregation. Low energy involvement enables the formation of stable transparent dispersions (smaller than 25 nm), which can be used for encapsulation of numerous bioactive compounds. 38

Microfluidization method
The technique involves use of a new, patented mixing technology employing high shear fluid device known as microfluidizer. In this process, the liquid is forced at speed up to 400 m/s through microchannels to an impingement area at high operating pressures. Cavitation and the accompanying shear and impact are accountable for the efficient particle size reduction within the "interaction chamber". The technique can be utilized on laboratory as well as production scale. 39

Membrane contactor technique
Membrane contactor is used to identify membrane systems that are employed to "Keep in contact" two phases. The lipid phase, at a temperature above its melting point is placed in a pressurized vessel. It is allowed to permeate through ceramic membrane pores under applied pressure to form small droplets. The aqueous phase, under continuous stirring, flow tangentially inside the membrane module, and brush away the droplets formed at the pore outlets. Cooling of the preparation to room temperature leads to the formation of lipid particles. Temperature of aqueous and lipid phase, aqueous phase tangential-flow velocity and pressure of lipid phase and membrane pore size are the process parameters affecting size of lipid nanocarriers. The benefits of this new process of membrane emulsification are commercial scalability and control on particle size by fitting optimized parameters. 40

Characterization of NLC
Appropriate techniques are required for characterizing physicochemical properties of NLC in order to ensure their performance, product quality and stability. Various evaluation parameters like particle morphology, interfacial properties, drug entrapment efficiency, crystallinity studies etc enlighten the workability of NLCs as drug delivery system.

Particle size measurement
Particle size of NLC is generally determined by photon correlation spectroscopy (PCS) using Zetasizer which works on Mie theory. Photon correlation spectroscopy (alias dynamic light scattering or quasi-elastic light scattering) is based on the measurement of the fluctuations in scattered light arising from Brownian motion. 41 It provides the average particle size (z-average) and polydispersity of the system as a measure of the particle size distribution. It characterizes particles of few nanometers to about 3 microns. Laser diffractometer (LD) can characterize a wide range from the nanometer to the micrometer range particles. This evaluation is based on the diffraction pattern depicting particle shape and size (explained by Fraunhofer theory). 1 In progression, combining Laser diffraction with Polarization intensity differential scattering technology results in the sizing of particles as small as 10 nm.
Data obtained is interpreted by calculating the volume distribution denoted by Dv (10), Dv(50), Dv (90).The parameter Dv (90) indicates the size point upto which 90% of cumulative volume of material in a given sample is contained. Similarly, Dv(50) value gives size point below which 50 % of the sample has the given size. In particle size determination, span is a parameter depicting polydispersity index (PI), i.e. how wide is size distribution of a nanoparticles population. It is the measure of particle homogeneity and its value lies between 0 and 1. A high span value indicates a wide distribution in sizes and a high polydispersity. [42][43][44] Theoretically, monodisperse populations indicates PI = 0. Therefore, PI measurement is essential to confirm the narrow size distribution of the particles. 45 (10) Dv (50) Another technique for particle size classification is by field flow fractionation (FFF) based on the size dependent migration and accumulation profile of particles on application of a perpendicular external field in a laminar flow. The classification into different size fractions can be realized by gravitation (sedimentation FFF), by thermophoresis in temperature gradients (thermal FFF), by electric fields (electrical FFF) or by hydrodynamic fields (cross flow FFF). 46 Other reported techniques used for particle size determination are dynamic ultramicroscopy, (characterization), ultrasonic spectroscopy, cryogenic transmission electron microscopy (cryo-TEM) analysis, electroacoustic mobility spectroscopy. Determining the particle size is of great importance in nanostructured formulations as the small particle size contributes to a greater interfacial area, which can then provide better drug partitioning and absorption at the skin surface. [47][48][49] Zeta potential Zeta potential (ZP) is a key factor for evaluation of the stability of nano dispersion. The ZP determination is based on particle electrophoretic mobility in aqueous medium. Zeta Potential characterizes the surface charge and gives the information about long term stability. At higher ZP the particle aggregation is less likely due to electric repulsion while dispersions with lower values tend to coagulate or flocculate, possibly leading to less stability. 50 Generally the zeta potential of dispersion should be either less than -30 mV or greater than +30 mV for electrostatic stabilization of NLC. 51 Zeta potential estimation can be done by Laser Doppler electrophoresis, using a Malvern ZetaSizer Nano ZS. By applying an electric field across the sample, particles with a zeta potential will migrate toward the electrode of opposite charge with a velocity relative to the magnitude of the zeta potential. The velocity is measured utilizing the technique of Laser Doppler anemometry, also known as Laser Doppler velocimetry. 52,53 The frequency shift of an incident laser beam caused by these moving particles is measured as particle mobility, and this is converted to the zeta potential according to the Henry equation. Zeta potential is influenced by factors like electrical conductivity, pH, and the nature of the reagents. 53

NLC morphology
Surface morphology of NLC can be observed by transmission and scanning electron microscopy (TEM, SEM), atomic force microscopy (AFM) and PCS. These techniques are tried and true for dimensional and structural characterization of NLCs. 13 Negative staining, freeze-fracture and vitrification by plunge freezing are different methods of sample preparation for TEM which can furnish different information about the colloidal particles. The sample is put on a gold or copper grid with defined mesh size grid and stained with heavy metal salt solution which provides high contrast in the electron microscope. After drying, the sample is viewed in the electron microscope where the Nanoparticles appear bright against the darker background of the stain. 54 Dehydration during sample preparation may lead to structural changes, thus original morphology of nano carriers may get altered. Cryo-transmission electron microscopy (cryo-TEM) and cryo-electron tomography (cryo-ET) is robust and indispensable tool to visualize colloidal drug delivery system. The methodology allows investigation of the sample in a vitrified frozen hydrated state, allowing visualization of nanoparticles basically as they exist in solution. 55 Sometimes use of surfactant in SEM imaging leads to artifacts due to formation of smooth camouflaging coating on particle surface. 56 AFM is a relatively simple and non-invasive technique that could be abused as a main imaging tool to measure & manipulate morphology and size of lipid nanoparticles. AFM offers an upper edge in characterizing heterogeneous materials and providing high grade data. 57 Samples for AFM requires fixation by removal of water, which may cause alteration in the status of emulsifier and polymorphic changes in lipids. 46 The technique does not utilize beams or radiations however a sharp-tipped scanning probe fixed to the free end of a spring like cantilever. Deflection, oscillation or shift in resonance frequency of cantilever motion are used to quantify interaction between the tip and surface of the specimen. 11

Entrapment efficiency
It is the influence on the release characteristics that determines the amount of drug loaded in NLC, a prime importance. Entrapment efficiency (Ee) defines the ratio between the weight of entrapped drug and the total weight of drug added to the dispersion. The amount of drug encapsulated per unit weight of the NLC is determined by ultrafiltration-centrifugation method. A known dispersion of NLCs is prepared and centrifugation is performed in a centrifuge tube mounted with an ultrafilter. After appropriate dilution the amount of free drug in supernatant is determined by spectrophotometer. 43 The entrapment efficiency in the NLCs is calculated by the following equation.

− * 100 %
where, Wa is the initial weight of drug used and Ws is the amount of drug determined in supernatant after separation of the lipid and aqueous phase. 58 Loading capacity Lc expresses the ratio between the entrapped drug and the total weight of the lipids. It is determined as follows: where Wl is the weight of lipid added in the formulation. 58 Liquid lipids create a disordered crystal matrix and consequently more space is available for loading large amount of drug, hence enhancing entrapment efficiency. Ee and Lc are dependent on several parameters, such as the lipophilic properties of the active pharmaceutical ingredient, the screening of the most appropriate lipid composition/ratio (solid/liquid lipids) and surfactant combination, as well as the production procedure used. 59

Crystallinity and polymorphism
The characterization of the crystallinity of the NLC components is important as the lipid matrix as well as the loaded drug may undergo a polymorphic transitional change leading to a possible undesirable drug leakage during storage. 60 The status of crystallinity of a particle also influences the encapsulation efficiency & release rates. 61 An increment in thermodynamic stability and lipid packing density, while a decrease in drug incorporation rate is observed in the following order 46 : Supercooled melt > alpha modification > beta' modification > beta modification To investigate crystallinity and polymorphic status, differential scanning calorimetry (DSC) and X-ray diffraction (XRD) experiments are performed.
DSC gives the heat lost or gained because of physical or chemical changes within a sample as a function of the temperature. DSC measurements reveal the status of lipid, melting and crystallization behavior of solid lipids in nanostructures. 62,63 DSC analysis is carried out on pure drug, pure lipids and nanoparticles. DSC characterization can illuminate NLC structure through the mixing behavior of solid lipids with liquid lipids. 64 The breakdown and fusion of the crystal lattice by heating or cooling the sample furnish exclusive information of polymorphism, crystal ordering, eutectic mixtures, glass transition processes and drug lipid interactions. 64,65 Recrystallization index (RI) is a parameter to perform comparative study of the crystallinity between the developed formulations. It can be calculated from the following formula: ΔHNLC ΔH bulk X Concentration of lipid × 100 where ΔH NLC =Melting enthalpy of 1 g NLC suspension, ΔH bulk = Melting enthalpy of 1 g bulk lipid, ΔH is given in J/g and the concentration is given by the percentage of lipid phase. 66 Gonullu et al determined Crystallization index (CI %) to determine the crystalline state of the drug in formulations.

= × 100
where, Ms = Melting enthalpy (J g-1) of lipid nanoparticles, Mp = Melting enthalpy (J g-1) of pure solid lipid, and ϓ represents solid lipid concentration (%) in nanoparticle dispersion. 62 An increase in amount of liquid lipid lowers the crystallinity and increases the defects in highly ordered structure of NLCs. The principle behind performing DSC rests on different enthalpy and melting point for different lipid changes. NLCs having a smaller size, therefore a higher surface area and employing more surfactants showed a decline in enthalpy and melting point of lipids. 10 XRD analysis is another useful technique to reveal polymorphic structural changes of compounds In XRD, the monochromatic beam of X-ray is diffracted at angles as per the type and arrangement of the atoms and space between the planes in the crystals. 60 Lipids has the ability to aggregate in a variety of ways giving rise to different polymorphic forms. This can be in form of micelles, lamellar phase, tubular arrangement or cubic phases. Wide angle and small angle X ray scattering techniques (WAXS, SAXS) are utilized to explore the layer arrangements, crystal structure, phase & polymorphic behavior of lipid and drug molecules. It also gives an idea regarding length of the short and long spacing of lipid lattice 46 and localization of drug in it. 67

Surface tension measurement
An increase in concentration of emulsifier lowers the interfacial tension of system due to the emulsification process. Surface tension of the lipid nanoparticles is often measured by the Wilhelmy plate method. The measurement of the contact angle is another method for detecting surface tension of the nanoparticulate systems. 11 Kibron instrument is a high precision, easy to use torsion balance instrument to measure surface tension of NLC. It employs the combination of maximum pull force technique and a unique ultrasensitive microbalance with Kibron's proprietary sensor. The instrument is at par in the technique of determining surface tension in comparison to platinum Wilhelmy plates or Du Noüy ring. 68

Evaluation of additional colloidal structures
In various cases, it has been observed that other colloidal structures like micelles, mixed micelles, liposomes, supercooled melts, accompany lipid nanoparticles. Evaluating these colloidal structures is quite a difficult task due to their size similarity, low resolution of PCS to image multimodal size distribution. Sample preparation may alter the equilibrium of the complex colloidal system. It may also require dilution of the dispersion with water which can lead to removal of surface active agent from nanoparticle surface. On this account technique like nuclear magnetic resonance (NMR) and electron spin resonance (ESR) are extremely useful tools for the simultaneous detection of colloidal structures and no sample preparation. These non-destructive methods are fruitful for examining dynamic phenomena and the presence of the nano oil compartments in colloidal lipid dispersions. 69 Magnetic resonance investigation NMR spectroscopy (1HNMR) and ESR are employed to enquire about structure of lipid nanoparticles and loaded drug. 70 The spectroscopy is used to judge mobility and interaction of oil molecules with solid lipid. This noninvasive technique permits a simple and rapid detection by virtue of width of signal. Proton relaxation times are associated with NMR line width. Mobile oil molecules will give small line widths with high amplitudes, whereas molecules with confined mobility give weak amplitudes and broad signals. Therefore, the technique is apt to evaluate the extent of immobilization. 46,71 Negative staining Negative staining is used to increase the electron opacity of the surrounding field. This technique uses salts of various heavy metals to determine the co-presence of colloidal lipid particles in dispersion by TEM. The test sample placed on a metal mesh grid is stained with solutions of heavy metal salts such as uranyl acetate, ammonium molybdate, sodium silicotungstate or sodium phosphotungstate that enhances the contrast. Sections are completely dried before attempting microscopic imaging. Presence of quick dried and stained artefacts makes it tedious to differentiate them from actual colloidal particles. The optical micrographs generated are of low resolution due to grain size stain. Negative staining electron microscopy may give three dimensional projections but problem may arise due to insufficient views on account of attachment of sample to the continuous carbon support in a preferred orientation. 10,72 Electron spin resonance (ESR) ESR investigation requires paramagnetic spin probes and no sample preparation. The spectra furnish information about molecular mobility and polarity inside the drug delivery system. This technique gives direct and noninvasive evaluation of the distribution of the spin probe between the aqueous and the lipid phases. 46 ESR spectra gives signal for paramagnetic lipids. This tool helps to understand the characteristic properties of nano compartments in lipid carriers. 71

Raman spectroscopy
It is an excellent tool to answer many questions about structural properties of lipids. The technique requires no preliminary steps and permit detections in the presence of water. 63 Fourier-transform infrared spectroscopy and Raman studies are conducted to detect drug-lipid interaction. Raman spectroscopy study excited molecular vibrations produced by a laser beam. The technique is used to map chemical and structural changes in molecules from their transition between two vibrational states. Raman spectroscopy is a useful tool to study packing, conformation and any changes in lipid chain arrangement after loading oils. 70

Structure of skin barrier
Skin, the largest multilayered organ of the body, with a surface area of 1.7-2.0 m 2 , is composed of several layers: stratum corneum (SC), the viable epidermis, the dermis and lower layers of adipose tissue. The SC, the superficial layer is the main roadblock of the skin. The SC comprises of corneocytes encompassed by lipid domains like long chain ceramides, free fatty acids, cholesterol, sterols and phospholoipds. 41 Corneocytes are covered by a cornified cell envelope (insoluble) which acts as a critical skin barrier formed by covalent cross-linking of component proteins such as involucrin, loricrin, and the small proline-rich protein. 73 The corneocytes are inserted in lipid lamellar locales, which are orientated parallel to the corneocyte surface. Since most drugs administered via skin dependably need to permeate along the tortuous pathway in these lipid locales, the organization and composition of the lipid component is viewed as imperative for the skin hindrance. 74,75 It is therefore, the lipids play an essential job in the skin hindrance, which makes their shared course of action in the lamellar areas, a key procedure in the development of the skin obstruction. Low pH of skin, presence of enzymes, skin hydration, diseased state and the transcutaneous concentration gradient are the other factors conceptualizing the barrier property of skin. Percutaneous absorption of topically applied agents also influenced by molecular weight, partition coefficient, formulation design, presence of penetration enhancer. 76 Beneficial role of NLC in transdermal drug delivery Transdermal drug delivery system is an established system since ages to attain diverse therapeutic objectives on different structural levels of skin (e.g. surface, epidermis, dermis and hypodermis). However, several problems associated with the conventional topical preparations have come in picture e.g. impermeability to skin barrier, limited efficacy and high frequency of application. The current focus of the researcher is quite related towards exploiting NLC for topical and dermal application, both in pharmaceutical as well as cosmetic sector. NLC are composed of biologically active and biodegradable lipids that show less toxicity and offer many favorable attributes such as adhesiveness, occlusion, skin hydration, lubrication, smoothness, emolliency, skin penetration enhancement, modified release, improvement of formulation appearance providing a whitening effect and offering protection of actives against degradation. 2 The key advantageous features offered by NLC that makes its role superior in transdermal drug delivery are as follows:

Skin permeation
Umpteen scientific literatures has stated about capability of NLC to control the rate of drug penetration into the skin, which limits the unwanted active absorption into blood circulation. 77 The smaller size of NLCs ensures a nearby contact with SC and can enhance the skin penetration of active compound. Investigators have reached consensus on the routes of nanoparticle penetration (Figure 2 pilosebaceous, and sweat gland pores. 57 Attributable to the hair follicle speaks to an invagination of the epidermis expanding profound into the dermis, which results in a more noteworthy genuine region for drug absorption. The hair follicle has turned into the most essential penetration pathway for nanoparticles among the appendageal routes. Besides, the hair follicles speak to an effective store for topically administered nanoparticles, which are normally extended deep into the tissue up to 2000 μm. Both permeation enhancement and sustained release are attainable by virtue of larger storage volume of hair follicle casts. Drug particle accumulate into the follicular casts followed by diffusion of drug from the nanocarriers into the skin. 78 Skin penetration of NLCs depend on their composition, and their physicochemical properties like size, aggregation, charge on particle surface, hydrophobicity, solubility of particles in the skin, solubilizing properties of particles towards the skin lipids, and whether particle possess film forming ability. 73

Transepidermal water loss (TEWL)
TEWL is the amount of water that passively evaporates through skin to the external environment due to water vapor pressure gradient on both sides of the skin barrier and is used to characterize skin barrier function. 79 TEWL loss measurements indicate damaged and weakened barrier function of the stratum corneum. An increase in TEWL marks a disruption of the stratum corneum and depletion of intercellular lipids. 41 Topical application of NLC depletes TEWL due to enhanced skin occlusion leading to skin hydration. It was observed that application of NLCs assisted to reduce moisture loss from the skin in comparison to the untreated skin. This owes to the ultrafine size of lipid nanocarriers actuating high surface area, and improved adhesive properties. The lipid particles forms a uniform compact film over the skin, hence inhibiting water evaporation from the skin. 80 Smaller the size of the particles, the reduced water evaporation from the skin. 41 It was found that the presence of a highly crystalline lipid in formulation decreases moisture loss from the stratum corneum effectively. 81 The presence of emollients (lecithin, propylene glycol) in NLC formulation helps to substitute for the depleted natural lipids present in the space between corneocytes in the stratum corneum to prevent excessive TEWL. 82

Skin occlusion
In general, lipid nanocarriers have peculiar epidermal occlusive characteristics which by inhibiting water evaporation could enhance bioactive penetration into the stratum corneum. Nanoparticles were found to be 15 times more occlusive than microparticles. Müller et al mentioned NLC as "invisible, penetration enhancing occlusive plastic foil. " 83 Scope of NLC occlusive feature depends on the following factors: • Particle size: Small particle size of the nanocarriers diminishes water evaporation from the skin. Occlusion factor of lipid micro particles of >1 µm diameter was found to be 10%, whereas lipid nanoparticles of 200 nm size showed 50% occlusion. 84 Wissing et al also depicts that formation of a monolayer film on skin require 4 mg of topical product with 4% lipid nanoparticles of approx size 200 nm. 81 This occlusive film as shown in Figure 3, being hydrophobic prevents dehydration of the skin, retards the penetration of UV filters in sunscreens and other whitening effects, lubrication/emolliency and control release properties. 37 • Crystallinity and concentration of lipids: High concentration of lipid (50%-60%) present in NLC formulation act as occlusive agent and is responsible for retaining the moisture in the stratum corneum. 41 It was found that high occlusivity can be attained with low melting lipids and highly crystalline particles. Formulation having particles size less than 400 nm and at least 35% lipid of high crystallinity was found to be most effective. An increase in oil content in NLC formula causes a decrement in occlusive factor. 81 Supercooled melts (noncrystalline nanoparticles) have no occlusive properties. 61 An enhanced skin hydration effect showed up due to the occlusive nature of NLCs. 84

Skin hydration and elasticity
Usually the human skin sustains a water content of 10%-20% of tissue dry weight. The surface lipid content and moisture state play an important role in the frictional properties of the skin. 41 Adequate hydration of skin slows down the signs of aging and protects from environmental damage. NLC fabricated from biocompatible and physiological lipids hold fast to the skin and produce an occlusive action expanding skin hydration. Lipid nanoparticle also retard moisture loss, hence loosen the corneocyte packing. The expanded intercorneocyte gap in turn leads to enhanced drug penetration. 85 NLCs could extend this superior hydration attribute due to its ultrafine size. Ascribed to small size lipid nanoparticles cling to lipid film of stratum corneum (hydrophobic interactions). The adsorbed film reconditions damaged skin and thus restores a protective thin film. 86 Owing to less particle size, the dimensions of the capillary channels of nanometer pores will be much smaller; thus, the hydrodynamic evaporation of water will decrease. 83,87 Loo et al depicted a high concentration of lipids in NLCs increase hydration more effectively as a consequence of occlusive layer. It is because of sliding of additional lipids into the intercellular space of the skin that assist in less TEWL. 82 Presence of humectants in formulation has an additional effect on hydration of SC by attracting moisture from dermis and air. Tichota et al formulated argan oil NLC-based hydrogel manifesting a synergistic effect on skin hydration (NLC occlusion plus argan oil hydration). 88 Skin hydration can be measured using a corneometer. The instrument works on the mechanism of measuring capacitance or conductance of a dielectric medium. As the hydration level of stratum corneum increases, its dielectric property alters. The method is simple, quick and hardly affected by skin residues. 82 It has been observed that skin hydration gets amplified with increased con centration of lipids. 41 Skin microrelief is used to evaluate skin-hydration efficacy of cosmetics, and could be assessed by measuring the parameters of roughness, scaling, smoothing, and wrinkling. 88 The moisturisation effect of a product leads to the increase in skin hydration, which contributes to wrinkle smoothing and enhancing the penetration of bioactives into specific skin layers. 82 Any alteration in the hydration level of the skin are measured in arbitrary units.

Stability of bioactives
One of the most important features of lipid nanoparticles is the ability to retard the chemical degradation of actives by photochemical, hydrolytic and oxidative pathways. Chemical stability of drug molecule stoutly depends on solid lipid matrix of lipid nanoparticles. Researchers remarked that the chemical stability of NLC fabricated with less crystalline solid lipid and lattice imperfection were improved due to increased drug arrangement within the lipid matrix. Solid state minimizes the exchange of actives with water phase (diffusional law by Einstein). 89 Moreover, the structure and good chemical stability of lipid itself must be reviewed. Hence, the selection of most suitable lipid during preformulation studies is quite important. It has been noted in research that actives that are incorporated in the imperfections at the less crystalline lipid matrix of NLC provide prolonged physical stability. 65 Table 3 displays a brief literature of drugs incorporated in NLC to increase stability.

Prolonged effect
Release profile from lipid nanoparticles is drastically influenced by type of lipid (solid or oil) used to formulate the vehicle, concentration of surfactant (or surfactants), solubility and concentration of active in the lipid matrix and method of formulating NLC. 77 NLC are produced by controlled mixing of solid lipids with spatially incompatible liquid lipids leading to special nanostructures with improved drug incorporation and release properties. Table 4 present a review of work done for extending drug release using NLC.

Skin targeting
Targeted drug delivery to the epidermal layer could display immense benefits over systemic administration on countless therapies. For the treatment of skin ailments like acne, a fungus infection or hair fall; the efficacy of topical drug is speculated by their potential to reach the desired site of action (specific skin layers) and remain there in therapeutic effective concentration for the appropriate time. Poor therapeutic effects & adverse reactions exhibited by conventional topical carriers can be conquered by formulating dermal NLC. 22 The strategy to target epidermis layer is favorable in respect of safety and as well as a rich network of antigen-presenting cells in epidermis eliciting a higher immune response. 78 Researchers are yet to be in unison about the fact that which particle property would result in superior skin targeting. It has been found that drug characteristics like size of particulate carrier, surface charge and composition material affect skin permeation and pharmacodynamics of encapsulated drugs. Degree of drug binding with plasma protein affect the epidermal targeting and penetration of particle deep into HF, on account of the capillary network around this structure, predominantly in the growth phase. Following accumulation in HF, nanoparticles can diffuse rapidly and continuously into the dermis or subcutaneous region enhancing epidermal targeting as well as local delivery of drug. 78 It was observed that adjusting the size of particulate carriers enables an intra-or transfollicular penetration ( Figure 4) and drug delivery. Particles of different sizes and structures accumulate in the hair follicle openings and penetrate along the follicular duct, when applied onto the skin. Particle-based drug delivery systems can be used to target specific regions within the follicular duct as penetration depths are counted upon the size of the particles and on the hair follicle type. The motion of the hair may likewise add to the penetration of nanoparticles of a size of 20 nm. 97 Lipids that resemble the composition of sebum may likewise advance follicular penetration as a high sebum/water partition coefficient is a basic characteristic for transport into HF. Epidermal targeting may also be assumed due to a dissolution or erosion of

Regulatory perspective
One of the significant grounds for the wide acceptance and commercial success of NLC is that these carriers present minor regulatory obstacles. The nanostructure lipid carriers employ lipids and emulsifiers with physiological, nontoxic, biodegradable and compatible profiles. All the ingredients used are acknowledged GRAS status by regulatory authorities or already have been accepted for encapsulating pharmaceutical or food active compounds. Still in any case, it is essential to use all the ingredients in safe and accepted range. Most of them are obtained or consist of ingredients occurring naturally in the human body i.e. fatty acid & glycerol. These are known to be well tolerated and reduce cytotoxic or adverse drug reaction. 22,77,83

Aceclofenac
Melt-emulsification and low-temperature solidification method Ultrasonication method or high-speed homogenizer method To formulate a controlledrelease drug delivery system for a prolonged period to satisfy the goals of the treatment of arthritis like reducing pain and inflammation 1. Oleic acid played an important role in the release of aceclofenac from the NLC dispersion. 2. Liquid lipid being located in the outer shell of the nanoparticles, leading to a drug-enriched shell that is related to burst release at the initial stage. 3. No significant effect of the method of preparation on the release of drug.

42
Amoitone B Emulsion-evaporation and low temperaturesolidification technology To construct an effective delivery system for Amoitone B to realize sustained release, thus prolong drug circulation time in body and improve the bioavailability.
1. When contacting the release solution, the superficial drug was freed quickly, leading to the burst release in the initial stage. 2. With the degradation and erosion of lipid matrix, the drug incorporated into nanoparticles core was released in a prolonged way.

95
Oridonin Emulsion-evaporation and low temperaturesolidification technique To prepare oridonin-loaded NLC, in which the Nanoparticles can be obtained in mild conditions easily without the need of any special equipment and the sustained drug release can also be achieved.
1. During emulsification, at first, most of the drug was dispersed in lipid droplets due to low solubility in water.
2. With increasing temperature and the existence of surfactant which increased drug solubility in the water, drug was migrated from the lipid phase to water phase. 3. During the cooling of the produced O/W nanoemulsion, a repartitioning of the drug into the lipid phase occurred. 4. When reaching the recrystallization temperature of the lipid, the solid lipid core was rapidly solidified to form a solid lipid core in which liquid lipid was randomly distributed.

Patent status of NLC
Over the past decade, nanostructure lipid carriers have been exploited for formulating a number of transdermal and cosmetic agents. Various patents have been granted for nano-lipid formulations. Table 5 presents a brief review of patents on the subject of original invention in the nanostructure lipid carriers.

Conclusion and Future Perspective
NLC provide a large influence to the enormous bars that are a prerequisite in formulating a stable drug delivery system. The application of nanocarriers in transdermal drug delivery has proclaimed a new domain in the drug delivery. NLCs are chemically and physically stable system with improved drug incorporation and increased bioavailability. The increasing interest of industry in lipid carrier system has been remarkably expanding the advancements in latest years. At present more than 30 commercial NLC formulations are accessible in the market containing drug and cosmetics ingredient. NLC as smarter generation of lipid nanoparticles are promising candidates to provide skin targeting along with occlusive effect and prolonged release. Lipid nanocarriers are seeking industrial attention due to qualified and validated scale up of technology, GRAS status of excipients and easy large scale production. Future NLC formulations can bring more prosperity to the lipid carrier system because of its umpteen superior characteristics over first generation systems. Future concern also involves assessment of toxicity and health hazard associated with nanostructures. More research in preclinical and clinical investigation will pave the way to success for nano-lipid structures. Achievement in this field can be conceived if pharmaceutical industry picks up the academia research to style this carrier system for various therapeutic and cosmetic agents.

Ethical Issues
Not applicable.