Tabriz University of Medical Sciences Advanced Pharmaceutical Bulletin 2228-5881 4 5 2014 12 30 Evaluating Antiproliferative and Antioxidant Activity of Marrubium crassidens 459 464 10.5681/apb.2014.068 EN Sanaz Hamedeyazdan Simin Sharifi Hossein Nazemiyeh Fatemeh Fathiazad Journal Article 10.5681/apb.2014.068 2014 04 14 Purpose: Naturally occurring substances as novel drugs in cancer therapy, at all times, represent a challenge to science since medicinal plants are proving to be brilliant sources of new chemopreventive agents. Methods: In the present study, methanol extract from aerial parts of Marrubium crassidens was assessed for its antiproliferative activity in the breast cancer cell line MCF-7 through MTT bioassay using cell viability and cytotoxicity indices. The antioxidant property of M. crassidens extract together with its phenolic and flavonoids content were evaluated, as well. Results: According to data obtained in the study, M. crassidens exhibited antiproliferative activity with a gradual rise in cytotoxicty effect setting out on 240µg/mL concentration of the extract. Moreover, the RC50 value for antioxidant activity of the extract was determined as 40µg/mL and values for the total phenolic and flavonoids were calculated as 512.64mg gallic acid equivalent and 212.73mg quercetin equivalent per 100g of dry plant material. Conclusion: Generally, the observed antiproliferative and antioxidant properties of M. crassidens could be certified to the high amounts of phenolic and flavonoid content detected in the extract.