Tabriz University of Medical Sciences Advanced Pharmaceutical Bulletin 2228-5881 3 1 2013 06 30 High Performance Liquid Chromatographic Analysis of Almotriptan Malate in Bulk and Tablets 183 188 10.5681/apb.2013.030 EN Petikam Lavudu Avula Prameela Rani Chepuri Divya Chandra Bala Sekharan Journal Article 10.5681/apb.2013.030 2012 10 30 Purpose: A simple RP-HPLC method has been developed and validated for the determination of almotriptan malate (ATM) in bulk and tablets. Methods: Chromatographic separation of ATM was achieved by using a Thermo Scientific C18 column. A Mobile phase containing a mixture of methanol, water and acetic acid (4:8:0.1 v/v) was pumped at the flow rate of 1 mL/min. Detection was performed at 227 nm. According to ICH guidelines, the method was validated. Results: The calibration curve was linear in the concentration range 5–60 µg/mL for the ATM with regression coefficient 0.9999. The method was precise with RSD <1.2%. Excellent recoveries of 99.60 - 100.80% proved the accuracy of the method. The limits of detection and quantification were found to be 0.025 and 0.075 µg/mL, respectively. Conclusion: The method was successfully applied for the quantification of ATM in tablets with acceptable accuracy and precision.