Tabriz University of Medical Sciences Advanced Pharmaceutical Bulletin 2228-5881 9 1 2019 02 21 The Study of HLA-G Gene and Protein Expression in Patients withRecurrent Miscarriage 70 75 10.15171/apb.2019.009 EN Elnaz Mosaferi https://orcid.org/0000-0002-3447-2861 Nazila Alizadeh Gharamaleki https://orcid.org/0000-0003-1485-3656 Laya Farzadi https://orcid.org/0000-0001-9969-0352 Jafar Majidi https://orcid.org/0000-0002-7343-7989 Zohreh Babaloo https://orcid.org/0000-0001-9398-3680 Tohid kazemi Mehrnoosh Ramezani https://orcid.org/0000-0003-1962-1870 Meraj Tabatabaei https://orcid.org/0000-0002-4749-1914 Hamid Ahmadi https://orcid.org/0000-0001-6641-2460 Leili Aghebati maleki https://orcid.org/0000-0002-0044-5961 Behzad Baradaran https://orcid.org/0000-0002-8642-6795 Journal Article 10.15171/apb.2019.009 2018 03 19 2018 09 29 Purpose: Although it has been frequently confirmed that HLA-G plays an important role in thereproduction and pregnancy, the pattern of HLA-G gene and its protein expression are rarelyaddressed in studies. Therefore we conducted this study in regard to evaluate the HLA-G geneand its protein expression in the women’s placenta with recurrent miscarriage.Methods: Placental samples were obtained from the women who were admitted for deliveryor abortion in Al Zahra and Taleghani hospitals, Tabriz, Iran. HLA-G gene expression wasdetermined by real-time polymerase chain reaction (PCR) and HLA-G protein expression wasassessed by western blotting and immunofluorescence staining in the tissue samples.Results: The results showed a significant decrease in the expression of gene and proteins ofHLA-G in the women with recurrent miscarriage compared to the control placental tissues.Conclusion: According to the obtained results, it was concluded that the decrement of HLA-Ggene and protein expressions are associated with recurrent miscarriage. Since there areconflicting results from other studies, it is suggested to conduct a more comprehensive similarstudy with greater sample size.