Tabriz University of Medical Sciences Advanced Pharmaceutical Bulletin 2228-5881 10 2 2020 02 18 Characterization and Prospective Applications of the Exopolysaccharides Produced by Rhodosporidium babjevae 254 263 10.34172/apb.2020.030 EN Rasool Mirzaei Seveiri https://orcid.org/0000-0002-7742-3701 Masoud Hamidi https://orcid.org/0000-0003-3847-4247 Cédric Delattre Hamid Sedighian Guillaume Pierre Babak Rahmani Sina Darzi Clément Brasselet Fatemeh Karimitabar Ali Razaghpoor Jafar Amani https://orcid.org/0000-0002-5155-4738 Journal Article 10.34172/apb.2020.030 2019 06 04 2019 12 08 Purpose: Due to the potential industrial and therapeutic applications of the yeast exopolysaccharides (EPSs), there has been an increasing demand to assess these biopolymers with improved characteristics. This study aimed to characterize the EPSs from Rhodosporidium babjevae (ATCC 90942 and IBRC-M 30088) as well as to evaluate their possible antioxidant, emulsifying and antiproliferative activities. Methods: Rhodosporidium babjevae was cultured for 5 days and following isolation of supernatant, EPSs precipitated with adding of cold absolute ethanol and freeze-dried. The EPSs chemical structure was determined by FT-IR, SEM, HPLC-SEC and GC-MS. Additionally the solubility, water holding capacity and emulsifying activity of EPSs were evaluated. In vitro, antioxidant activity was investigated against DPPH, superoxide and hydroxyl radicals. Finally the EPSs consequence on the cell proliferation of human breast adenocarcinoma (MCF-7) and Madin-Darby canine kidney (MDCK) cell lines was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. Results: R. babjevae excreted 1.6±0.2 g/L of the EPSs. The EPSs had three fractions with molecular weights of 1.02 ×106 , 5×105 and 2×105 Da. Mannose and glucose were found as the main monosaccharides of the EPSs (84:16 mol%, respectively). The EPSs exhibited emulsifying activity on sun flower oil. The scavenging activities were found to be dose-dependent and higher than hyaluronic acid. Significant difference among the EPSs treatments on the proliferation of MCF-7 and MDCK cell lines was not observed (P>0.05). Conclusion: These results show the interesting potential of the EPSs from R. babjevae as biocompatible compounds for using in food and pharmaceutical fields.