Tabriz University of Medical Sciences Advanced Pharmaceutical Bulletin 2228-5881 12 1 2022 01 01 Isothermal Amplification of Nucleic Acids Coupled with Nanotechnology and Microfluidic Platforms for Detecting Antimicrobial Drug Resistance and Beyond 58 76 10.34172/apb.2022.004 EN Seyedeh Zahra Alamolhoda https://orcid.org/0000-0003-1184-7808 Nosratollah Zarghami Houman Kahroba Ahmad Mehdipour Mohammad Pourhassan-Moghaddam Rana Jahanban-Esfahlan https://orcid.org/0000-0002-5119-252X Morteza Milani https://orcid.org/0000-0002-9822-703X REVIEW 10.34172/apb.2022.004 2020 05 03 2021 01 29 Antibiotic resistance is one of the serious health-threatening issues globally, the controlof which is indispensable for rapid diagnosis and treatment because of the high prevalenceand risks of pathogenicity. Traditional and molecular techniques are relatively expensive,complex, and non-portable, requiring facilities, trained personnel, and high-tech laboratories.Widespread and timely-detection is vital to the better crisis management of rapidly spreadinginfective diseases, especially in low-tech regions and resource-limited settings. Hence, the needfor inexpensive, fast, simple, mobile, and accessible point-of-care (POC) diagnostics is highlydemanding. Among different biosensing methods, the isothermal amplification of nucleic acidsis favorite due to their simplicity, high sensitivity/specificity, rapidity, and portability, all becausethey require a constant temperature to work. Isothermal amplification methods are utilizedfor detecting various targets, including DNA, RNA, cells, proteins, small molecules, ions, andviruses. In this paper, we discuss various platforms, applications, and potentials of isothermalamplification techniques for biosensing of antimicrobial resistance. We also evaluate thepotential of these methods, coupled with the novel and rapidly-evolving platforms offered bynanotechnology and microfluidic devices.