Paulo César Venâncio
1, Sidney Raimundo Figueroba
2*, Bruno Dias Nani
2, Luiz Eduardo Nunes Ferreira
2, Bruno Vilela Muniz
2, Fernando de Sá Del Fiol
3, Adilson Sartoratto
4, Edvaldo Antonio Ribeiro Rosa
5, Francisco Carlos Groppo
21 Department of Exact Sciences, Technical School of Limeira, Cotil, UNICAMP, Limeira, São Paulo, Brazil.
2 Department of Physiological Sciences, Piracicaba Dental School, UNICAMP, Piracicaba, São Paulo, Brazil.
3 Department of Pharmacological Sciences, School of Pharmacy of Sorocaba, UNISO, Sorocaba São Paulo, Brazil.
4 Research Center for Chemistry, Biology and Agriculture, CPQBA, UNICAMP, Paulínia, São Paulo, Brazil.
5 Xenobiotics Research Unit, PUC-Paraná, Curitiba, Paraná, Brazil.
Abstract
Purpose: This study observed the effect of garlic extracts and amoxicillin against an induced staphylococcal infection model. MIC and MBC were also obtained for aqueous extracts of Allium sativum (Asa) and Allium tuberosum (Atu) against Staphylococcus aureus penicillin-sensitive (PSSA - ATCC 25923) and MRSA (ATCC 33592).
Methods: Granulation tissues were induced in the back of 205 rats. After 14 days, 0.5 mL of 108 CFU/mL of PSSA or MRSA were injected inside tissues. After 24h, animals were divided: G1 (Control) – 0.5 mL of NaCl 0.9%; G2 – Asa 100 mg/kg or 400mg/kg; G3 – Atu 100 mg/kg or 400 mg/kg; G4 – amoxicillin suspension 50 mg/kg, considering PSSA infection; and G5 (Control) - 0.5 mL of NaCl 0.9%; G6 – Asa 400mg/kg; G7 – amoxicillin 50 mg/kg; and G8 - Asa 400 mg/kg + amoxicillin 50 mg/kg for MRSA. All treatments were administered P.O. every 6h. Animals were killed at 0, 6, 12 and 24h. Samples were spread on salt-mannitol agar. Colonies were counted after 18 h at 37 °C. Atu was not able to inhibit or kill PSSA and MRSA. Considering Asa, MIC and MBC against PSSA were 2 mg/mL and 4 mg/mL, respectively; and 16 mg/mL and 64 mg/mL against MRSA.
Results: No effect was observed in vivo for control, Asa 100 mg/kg and Atu 100 mg/kg, while amoxicillin, Atu 400 mg/kg and Asa 400 mg/kg decreased PSSA counts in all-time points. No effect of any group against MRSA was observed at any time.
Conclusion: Thus, A. sativum and A. tuberosum were able to reduce PSSA infection, but not MRSA infection.