Zead Helmi Mahmoud Abudayeh
1*, Khaldun Mohammad Al Azzam
2*, Ahmad Naddaf
1, Uliana Vladimirovna Karpiuk
3, Viktoria Sergeevna Kislichenko
41 Faculty of Pharmacy, Isra University, 11622 Amman, Jordan.
2 Department of Pharmaceutical Chemistry, Pharmacy Program, Batterjee Medical College for Sciences and Technology (BMC), 21442 Jeddah, Kingdom of Saudi Arabia.
3 Department of Pharmacognosy and Botany, National Medical University is the Name of O.O.Bogomolets, Ukraine.
4 National University of Pharmacy, Kharkiv, Ukraine.
Abstract
urpose: To separate and quantify four major saponins in the
extracts of the skin and the endosperm of seeds of horse chestnut (Aesculus
hippocastanum L.) using ultrasonic solvent extraction followed by a high
performance liquid chromatography-diode array detector (HPLC-DAD) with positive
confirmation by thin layer chromatography (TLC).
Methods: The saponins: escin Ia, escin Ib, isoescin Ia and
isoescin Ib were extracted using ultrasonic extraction method. The optimized
extraction conditions were: 70% methanol as extraction solvent, 80 C as
extraction temperature, and the extraction time was achieved in 4 hours. The
HPLC conditions used: Zorbax SB-ODS-(150 mm × 2.1 mm, 3 m) column,
acetonitrile and 0.10% phosphoric acid solution (39:61 v/v) as mobile phase,
flow rate was 0.5 mL min-1 at 210 nm and 230 nm detection. The
injection volume was 10 L, and the separation was carried out isothermally at
30 C in a heated chamber.
Results: The results indicated that the
developed HPLC method is simple, sensitive and reliable. Moreover, the content
of escins in seeds decreased by more than 30% in endosperm and by more than 40%
in skin upon storage for two years.
Conclusion: This assay can be readily utilized
as a quality control method for horse chestnut and other related medicinal
plants.