Logo-apb
Submitted: 10 Feb 2013
Revision: 24 Feb 2013
ePublished: 20 Aug 2013
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

Adv Pharm Bull. 2013;3(2): 289-293.
doi: 10.5681/apb.2013.047
PMID: 24312850
PMCID: PMC3848207
Scopus ID: 84885743023
Plum Print visual indicator of research metrics
  • Citations
    • Citation Indexes: 19
  • Captures
    • Readers: 36
see details
  Abstract View: 1346
  PDF Download: 1210

Original Research

Development and Application of an HPLC Method for Erlotinib Protein Binding Studies

Soheila Bolandnazar, Adeleh Divsalar, Hadi Valizadeh, Arash Khodaei, Parvin Zakeri-Milani*
*Corresponding Author: Email: PZAKERI@TBZMED.AC.IR

Abstract

Purpose: The aim of the present study was to develop a simple and rapid reversed-phase high performance liquid chromatographic method with UV detection for erlotinib hydrochloride quantification, which is applicable for protein binding studies. Methods: Ultrafilteration method was used for protein binding study of erlotinib hydrochloride. For sample analysis a simple and rapid reversed-phase high performance liquid chromatographic method with UV detection at 332 nm was developed. The mobile phase was a mixture of methanol, acetonitril and potassium dihydrogen phosphate buffer (15:45:40 %v/v) set at flow rate of 1.3 ml/min. Results: The run time for erlotinib hydrochloride was approximately 6 minutes. The calibration curve was linear over the range of 320-20000 ng/ml with acceptable intra- and inter-day precision and accuracy. The intra-day and inter-day precisions were less than 10% and the accuracies of intra and inter-day assays were within the range of 97.20-104.83% and 98.8-102.2% respectively. Conclusion: Based on the obtained results, a simple, accurate and precise reversed-phase isocratic HPLC method with UV detection has been optimized and validated for the determination of erlotinib hydrochloride in biological samples.
Citations
  • Citation Indexes: 19
Captures
  • Readers: 36
First Name
Last Name
Email Address
Comments
Security code


Abstract View: 1347

Your browser does not support the canvas element.


PDF Download: 1210

Your browser does not support the canvas element.