Ali Golestan
1, Abbas Ghaderi
2, Zahra Mojtahedi
2*1 Autophagy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
2 Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract
Purpose:
N-myc downstream-regulated gene 2 (NDRG2) is frequently down-regulated in
cancer, and plays an important role in the control of tumor growth and
metastasis. Its manipulation has been suggested as a therapy in cancer. Here,
we examined the outcome of NDRG2 overexpression on proliferation, invasion,
migration and MMP activity of HCT116 colorectal cancer cell line.
Methods: The
HCT116 cell line (human colorectal cancer) was transfected with
pCMV6-AC-GFP-NDRG2.
2,5diphenyltetrazolium bromide (MTT) assay was used to detect cell
proliferation. The invasion and migration of the transfected cells were
examined through transwell chambers while the MMP-9 activity was detected by
the ability of the cells to digest gelatin.
Results: Overexpression
of NDRG2 by stable NDRG2 transfection decreased cell proliferation, migration
and invasion ability, along with decreasing MMP-9 activity.
Conclusion: Our data indicate that
NDRG2 overexpression can suppress several aspect of tumorigenesis. Further
investigations are necessitated to verify if NDRG2 molecule can be a
therapeutic target in colorectal cancer.