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Adv Pharm Bull. 2017;7(4): 661-664.
doi: 10.15171/apb.2017.080
PMID: 29399558
PMCID: PMC5788223
Scopus ID: 85043374839
  Abstract View: 1825
  PDF Download: 1298

Short Communication

Effects of NDRG2 Overexpression on Metastatic Behaviors of HCT116 Colorectal Cancer Cell Line

Ali Golestan 1, Abbas Ghaderi 2, Zahra Mojtahedi 2*

1 Autophagy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
2 Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran.
*Corresponding Author: Email: mojtahediz@sums.ac.ir

Abstract

Purpose: N-myc downstream-regulated gene 2 (NDRG2) is frequently down-regulated in cancer, and plays an important role in the control of tumor growth and metastasis. Its manipulation has been suggested as a therapy in cancer. Here, we examined the outcome of NDRG2 overexpression on proliferation, invasion, migration and MMP activity of HCT116 colorectal cancer cell line. Methods: The HCT116 cell line (human colorectal cancer) was transfected with pCMV6-AC-GFP-NDRG2. 2,5diphenyltetrazolium bromide (MTT) assay was used to detect cell proliferation. The invasion and migration of the transfected cells were examined through transwell chambers while the MMP-9 activity was detected by the ability of the cells to digest gelatin. Results: Overexpression of NDRG2 by stable NDRG2 transfection decreased cell proliferation, migration and invasion ability, along with decreasing MMP-9 activity. Conclusion: Our data indicate that NDRG2 overexpression can suppress several aspect of tumorigenesis. Further investigations are necessitated to verify if NDRG2 molecule can be a therapeutic target in colorectal cancer.
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Submitted: 30 Jan 2017
Revision: 03 Jul 2017
Accepted: 16 Jul 2017
ePublished: 31 Dec 2017
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