Javad Tajkey
1, Alireza Biglari
2*, Bohlol Habibi Asl
1*, Ali Ramazani
3, Saeideh Mazloomzadeh
41 Department of Pharmacology, School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Department of Genetics and Molecular Medicine, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
3 Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
4 Department of Epidemiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Abstract
Purpose: Comparison between the efficacy of ceftriaxone and monocytes on
improvement of neuron protection and functional recovery after spinal cord
injury (SCI) in rat.
Methods: Rats were randomly
divided into three groups of ten. Spinal cord injury was performed on rats
under general anesthesia using the weight dropping method. Ceftriaxone was
injected intraperitoneally 200 mg/kg/day for seven days after SCI. Monocytes
were injected 2 × 105 cells 4 days after SCI. Hind
limb motor function was assessed using the Basso, Beattie and Bresnahan (BBB)
scale. Corticospinal tract (CST) axons were traced by injection of biotin
dextran amine (BDA) into the sensorimotor cortex.
Results: There were statistically significant differences in BBB
scores in ceftriaxone in comparison to both monocytes receiving and control
groups. On the other hand there were statistically significant differences in
axon counting in both ceftriaxone and monocytes receiving groups in comparison
to control group.
Conclusion: Our findings suggest that ceftriaxone improves
functional recovery more effective than monocytes in rats after SCI. These
results are from an experimental model and validation is required for further
investigation.