Abstract
Purpose: Human bone marrow-derived mesenchymal cell (hBMC) reactions to 316L
stainless steel (316L-SS) have never been
evaluated. The objective of this study was to assess cell viability and
interleukin-6 expression of hBMC cultures upon treatment with a 316L-SS
implant.
Methods: A cytotoxicity analysis was
conducted with a 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium (MTT) assay after
a period of 24, 48 and 72 hours of incubation. Expression of interleukin-6 was
measured using enzyme-linked immunosorbent assay
(ELISA).
Results: Cell viability measurement
was performed via IC50
formula. All treatment group showed a > 50 % cell viability with a range of
56,5 - 96,9 % at 24 hours, 51,8-77,3% at 48 hours and 70,1- 120 % at 72 hours.
Interleukin-6 expression was downregulated subsequent to treatment with 316L-SS
compared to the control group.
Conclusion: We found that 316L-SS did not
exhibit toxicity towards
hBMC culture.