Adv Pharm Bull. 2017;7(2): 335-338.
doi: 10.15171/apb.2017.040
PMID: 28761837
PMCID: PMC5527249
Scopus ID: 85021405698
  Abstract View: 1663
  PDF Download: 793

Short Communication

Human Bone Marrow-Derived Mesenchymal Cell Reactions to 316L Stainless Steel: An in Vitro Study on Cell Viability and Interleukin-6 Expression

Iwan Budiwan Anwar 1,2*, Asep Santoso 2 ORCID logo, Eko Saputra 1,3, Rifky Ismail 3, J. Jamari 3, Emile Van der Heide 1

1 Laboratory for Surface Technology and Tribology, Faculty of Engineering Technology, University of Twente Drienerloolaan 5, Postbox 217, 7500 AE, Enschede, The Netherlands.
2 Orthopaedic and Traumatology Department, Prof. Dr. R. Soeharso Orthopaedic Hospital, Jl. A. Yani Pabelan, Surakarta 57162, Indonesia.
3 Laboratory for Engineering Design and Tribology, Department of Mechanical Engineering, Diponegoro University, Jl. Prof. Soedharto, Tembalang, Semarang 50275, Indonesia .


Purpose: Human bone marrow-derived mesenchymal cell (hBMC) reactions to 316L stainless steel (316L-SS) have never been evaluated. The objective of this study was to assess cell viability and interleukin-6 expression of hBMC cultures upon treatment with a 316L-SS implant.

Methods: A cytotoxicity analysis was conducted with a 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium (MTT) assay after a period of 24, 48 and 72 hours of incubation. Expression of interleukin-6 was measured using enzyme-linked immunosorbent assay (ELISA).

Results: Cell viability measurement was performed via IC50 formula. All treatment group showed a > 50 % cell viability with a range of 56,5 - 96,9 % at 24 hours, 51,8-77,3% at 48 hours and 70,1- 120 % at 72 hours. Interleukin-6 expression was downregulated subsequent to treatment with 316L-SS compared to the control group.

Conclusion: We found that 316L-SS did not exhibit toxicity towards hBMC culture.

Keywords: 316L stainless steel, Human bone marrow-derived mesenchymal cells, Cell viability, Interleukin-6
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Submitted: 25 Apr 2017
Revision: 11 Jun 2017
Accepted: 12 Jun 2017
ePublished: 30 Jun 2017
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