Atinderpal Kaur
1, Sonal Gupta
1, Amit Tyagi
2, Rakesh Kumar Sharma
3, Javed Ali
4, Reema Gabrani
1, Shweta Dang
1*1 Department of Biotechnology, Jaypee Institute of Information Tehnology, A-10, Sector 62, Noida, UP 201307, India.
2 Department of Nuclear Medicine, Institute of Nuclear Medicine and Allied Sciences, Brig SK Mazumdar Marg, Delhi, 110054, India.
3 Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, Brig SK Mazumdar Marg, Delhi, 110054, India.
4 Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi, 110062, India.
Abstract
Purpose: A nanoemulsion based gel containing
Polyphenon 60 (P60) and cranberry (CRB) has been developed to deliver via
intravaginal route for the treatment of urinary tract infection.
Methods: Polyphenon 60 and cranberry were loaded in
a single nanoemulsion gel (NBG) by ultra-sonication method and characterized
for particle size, rheological properties, in vitro release and growth curve
analysis. P60+CRB NBG were radiolabelled using technetium pertechnetate (99mTc)
to perform in vivo pharmacokinetic
studies in animals.
Results: The finalized NE had a droplet size of
58±1 nm. In vitro release of 90.92 ±
0.6% in 8 hr for P60 and 99.39 ± 0.5% in 6 hr for CRB was observed in simulated
vaginal fluid. Growth curve of E. coli
indicated the inhibitory action of nanoemulsion based gel at the fifth hour of
inoculation. Gamma scintigraphy studies on female Sprague-Dawley rats showed
transport of nanoemulsion based gel from the vaginal cavity into the systemic
circulation. Further, biodistribution studies with radiolabelled P60+CRB NBG
showed significant higher uptake of radiolabelled actives by kidney (3.20±0.16)
and urinary bladder (3.64±0.29), when administered intravaginally.
Conclusion: The findings suggested 99mTc-P60+CRB
NBG can potentially be transported through vaginal cavity and reach the target
organs and showed effective distribution in organs affected in urinary tract
infection