Abstract
Purpose: Terminal deoxynucleotidyl transferase(TdT) is a DNA polymerase that is present in immature pre-B and pre-T cells.
TdT inserts N-nucleotides to the V (D) J gene segment during rearrangements of
genes, therefore, it plays a vital role in the development and variation of the immune
system in vertebrates. Here we evaluated the
relationship between cytokines like interleukin-2 (IL-2),
interleukin-7 (IL-7), and interleukin-15 (IL-15) and
TdT expression in cord blood mononuclear cells and also effect of inhibition in
the expansion of B and T cells derived from cord blood.
Methodes: The cord blood
mononuclear cells were cultured with different combination of cytokines for
21days, which they were harvested in definite days (7, 14 and 21) and evaluated
by flow cytometry.
Results: Our data
indicated that TdT expression increased in cord
blood mononuclear cells using immune cell key cytokines without being dependent
on the type of cytokines. TdT inhibition reduced both the expansion of B and T
cells derived from cord blood and also declined the apoptosis and proliferation.
Considered together, TdT played an important role in the control of the
expansion of B and T cells derived from cord blood.
Conclusion: considered together, it was observed that TdT
expression was increased by cytokines and TdT inhibition not only reduced B and
Tcells derived from cord blood, but it also affected the rate of apoptosis and
proliferation.