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Adv Pharm Bull. 2015;5(4): 463-469.
doi: 10.15171/apb.2015.063
PMID: 26819917
PMCID: PMC4729352
Scopus ID: 84949685207
  Abstract View: 2200
  PDF Download: 816

Original Research

Short-Term Treatment with Silymarin Improved 6-OHDA-Induced Catalepsy and Motor Imbalance in Hemi-Parkisonian Rats

Rasool Haddadi 1, Shahla Eyvari Brooshghalan 2, Safar Farajniya 2, Alireza Mohajjel Nayebi 2,3*, Hamdolah Sharifi 4

1 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Hamadan University of Medical Science, Hamadan, Iran.
2 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Clinical Psychiatry Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Department of Pharmacology, Faculty of Pharmacy, Urmia University of Medical Science, Urmia, Iran.
*Corresponding Author: Email: nayebia@tbzmed.ac.ir

Abstract

 

Purpose: Parkinson’s disease (PD) is a common neurodegenerative disorder characterized by disabling motor abnormalities, which include tremor, muscle stiffness, paucity of voluntary movements, and postural instability. Silymarin (SM) or milk thistle extract, is known to own antioxidative, anti-apoptotic, anti-inflammatory and neuroprotective effects. In the present study, we investigated the effect of intraperitoneal (i.p) administration of SM, on 6-OHDA-induced motor-impairments (catalepsy and imbalance) in the rats.

Methods: Experimental model of PD was induced by unilateral infusion of 6-hydroxydopamine (6-OHDA; 8 μg/2 μl/rat) into the central region of the substantia nigra pars compacta (SNc). Catalepsy and motor coordination were assessed by using of bar test and rotarod respectively.

Results: The results showed a significant (p<0.001) increase in catalepsy of 6-OHDA-lesioned rats whereas; in SM (100, 200 and 300 mg/kg, i.p for 5 days) treated hemi-parkinsonian rats catalepsy was decreased markedly (p<0.001). Furthermore, there was a significant (p<0.001) increase in motor-imbalance of 6-OHDA-lesioned rats. SM improved motor coordination significantly (p<0.001) in a dose dependent manner and increased motor balance.

Conclusion: In conclusion, we found that short-term treatment with SM could improve 6-OHDA-induced catalepsy and motor imbalance in rats. We suggest that SM can be used as adjunctive therapy along with commonly used anti-parkinsonian drugs. However, further clinical trial studies should be carried out to prove this hypothesis.

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Submitted: 11 Mar 2015
Revision: 26 May 2015
Accepted: 13 Jun 2015
ePublished: 30 Nov 2015
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