Chusnul Chotimah
1, 2, Sudjadi Sudjadi
1, Sugeng Riyanto
1, Abdul Rohman
1*1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
2 The National Agency of Drug and Food Control, district of Yogyakarta, Indonesia.
Abstract
Purpose: Analysis
of drugs in multicomponent system officially is carried out using
chromatographic technique, however, this technique is too laborious and
involving sophisticated instrument. Therefore, UV-VIS spectrophotometry coupled
with multivariate calibration of partial least square (PLS) for quantitative
analysis of metamizole, thiamin and
pyridoxin is developed in the presence of cyanocobalamine without any separation step.
Methods: The
calibration and validation samples are prepared. The calibration model is prepared
by developing a series of sample mixture consisting these drugs in certain
proportion. Cross validation of
calibration sample using leave one out technique is used to identify the
smaller set of components that provide the greatest predictive ability. The evaluation of
calibration model was based on the coefficient of determination (R2) and root mean square error of calibration (RMSEC).
Results: The
results showed that the coefficient of determination (R2) for the relationship
between actual values and predicted values for all studied drugs was higher
than 0.99 indicating good accuracy. The RMSEC values obtained were relatively
low, indicating good precision. The accuracy and presision results of developed
method showed no significant difference compared to those obtained by official method
of HPLC.
Conclusion:
The developed method (UV-VIS spectrophotometry
in combination with PLS) was succesfully used for analysis of metamizole, thiamin and pyridoxin in
tablet dosage form.