Zahra Mardhiah Adib
1, Saeed Ghanbarzadeh
2, Maryam Kouhsoltani
3, Ahmad Yari Khosroshahi
4, Hamed Hamishehkar
5*1 Research Center for Pharmaceutical Nanotechnology and Students’ Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Zanjan Pharmaceutical Nanotechnology Research Center, Department of Pharmaceutics, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
3 Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Biotechnology Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
5 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
Purpose: In the present study the effect of particle size, as a substantial
parameters in skin penetration, on the deposition depth and rate of SLNs in different layers of skin was explored.
Methods: SLNs in different particle
size ranges (80, 333 and 971 nm) made of Precirol as solid lipid were prepared
using hot melt homogenization technique and pigmented by Rhodamine B to be able
to be tracked in the skin under inspection of fluorescent microscopy. After 0.5
h, 3 h, 6 h and 24 h of SLNs administration on rat skin, animals were
sacrificed and exercised skins were sliced by a freeze microtome. SLNs were
monitored in the skin structure under fluorescence microscope.
Results: The size of SLNs played a
crucial role in the penetration to deep skin layers. The sub100 nm size range
of SLNs showed the most promising skin penetration rate and depth mainly via
hair follicles.
Conclusion: The results of the present
study indicated that the selection of an appropriate size of particles may be a
valuable factor impacting the therapeutic outcomes of dermal drug
administration.