Maryam Mohammadi
1,2, Akram Pezeshki
3,2, Mehran Mesgari Abbasi
4, Babak Ghanbarzadeh
2, Hamed Hamishehkar
4*1 Biotechnology Research Center and Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz,Tabriz, Iran.
3 Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences,Tabriz, Iran.
4 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
Purpose: Nanostructured
lipid carriers (NLCs) composed of solid lipid and oil are a new generation of
lipid nanoparticles which have exhibited some merits over traditional used
lipid nanoparticles in fortifying food and beverages and nutraceuticals
delivery systems such as liposomes and solid lipid nanoparticles.
Methods: In this study,
Precirol and Compritol as solid lipids, Miglyol and Octyloctanoat as liquid
lipids, Tween80, Tween20 and Poloxamer407 as surfactants were used to prepare
vitamin D3-loaded NLC dispersion using hot homogenization method.
The particle size and size distribution for all formulations were evaluated by
immediately after production and during a storage period of 60 days.
Results: The
Precirol-based NLC showed superiority over Compritol-based NLC in the point of
physical stability. Results clearly suggested that an optimum concentration of
3% of Poloxamer407 or 2% of Tween20 was sufficient to cover the surface of
nanoparticles effectively and prevent agglomeration during the homogenization
process. Octyloctanoat was introduced for the first time as a good substituent
for Miglyol in the preparation of NLC formulations. The vitamin D3 Intestinal
absorption enhanced by the incorporating in NLCs.
Conclusion: It was concluded that NLC showed a promising approach
for fortifying beverages by lipophilic nutraceuticals such as vitamin D.