Abstract
Purpose: The study on the interaction
between small molecules and DNA has been very useful for investigating the
structure and physical properties of DNA, elucidating the damage mechanism of
DNA and significant in the design of new drugs targeted to DNA. This article
describes an interaction of native calf thymus DNA
(ctDNA) with a new 3, 4-dihydropyrano[c]chromene derivative,
2-amino-4-(4- chlorophenyl)-5-oxo-4H, 5H-pyrano-[3, 2-c]
chromene-3-carbonitrile (4-CC) by using spectroscopic and
viscometric techniques.
Methods: The interaction between
4-CC and ctDNA is realized from the UV absorption spectrophotometry, viscosimetry,
circular dichroism and fluorescence spectroscopic techniques which shows that the successive interaction of 4-CC with
ctDNA occurs.
Results: The experimental
results revealed that 4-CC can interact with DNA through non- intercalative
mode and the intrinsic binding constant (Kb) for 4-CC with DNA was
estimated to 2.37 (±0.001) ×103 M-1. Methylene blue (MB)
displacement studies revealed that 4-CC did not have any effect on MB bound DNA
which is indicative of groove binding mode. Furthermore, 4-CC induces
detectable changes in the CD spectrum of ctDNA as well as changes in its
viscosity study corroborate the above experimental results.
Conclusion: These results further advance our knowledge on the molecular
aspects on the interaction of 4-CC to nucleic acids.