Abstract
Purpose: Mesenchymal
stem cells (MSCs) are multipotent cells and recent findings suggest
immunomodulatory effect of them on immune cells including T cells and dendritic
cells (DCs). DCs are the most potent antigen presenting cells. It seems because
of immunoregulatory properties of MSCs, they can affect the maturation and
differentiation of DCs. DCs express a kind of surface receptors called
toll-like receptors (TLRs) and play a key role in maturation process and
activation of DCs. The aim of this study was to evaluate expression of TLR2 and
TLR4 on DCs after exposure to mesenchymal stem cell’s supernatant in culture
media containing LPS and devoid of it.
Methods: In this experimental study, MSCs and DCs were extracted
from adult Balb/c mouse bone marrow and spleen, respectively. MSCs supernatant
were collected 24 and 48 h after 5th passage, and in adjusted with
DCs culture. Isolated DCs were co-cultured with MSCs supernatant, incubation
time were 24 and 48 hours. mRNA levels of TLR2 and TLR4 were evaluated using
real time PCR technique.
Results: The results demonstrated that although, expressions of
these two receptors were up-regulated in culture media lacking LPS in
comparison with the control group but the increase was not significant. There
were no significant associations between LPS stimulated DCs with and without
MSCs supernatants.
Conclusion: According to the results presented here, it appears
that TLR2 and TLR4 gene expressions on the DCs are not affected by MSCs
supernatant.