Parisa Jourghanian
1, Solmaz Ghaffari
2,3*, Mehdi Ardjmand
4, Setareh Haghighat
5, Mahdieh Mohammadnejad
61 Department of Chemical Engineering, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
2 Department of Medical Nanotechnology, Faculty of Advanced Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University, (IAUPS), Tehran, Iran.
3 Young Researchers and Elite Club, Pharmaceutical Sciences Branch, Islamic Azad University, (IAUPS), Tehran, Iran.
4 Department of Chemical Engineering, South Tehran Branch, Islamic Azad University, Tehran, Iran.
5 Department of Microbiology, Faculty of Advanced Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran.
6 Quality Control Department, Tofigh Daru Research and Engineering Co. Tehran, Iran.
Abstract
Purpose: curcumin is poorly water soluble drug with low
bioavailability. Use of lipid systems in lipophilic substances increases
solubility and bioavailability of poorly soluble drugs. The aim of this study
was to prepare curcumin loaded Solid Lipid Nanoparticles (SLNs) with high
loading efficiency, small particle size and prolonged release profile with
enhanced antibacterial efficacy.
Methods: to synthesize stable SLNs, freeze- Drying was done
using mannitol as cryoprotectant. Cholesterol was used as carrier because of good
tolerability and biocompatibility. SLNs were prepared using high pressure
homogenization method.
Results: optimized SLNs had 112 and 163 nm
particle size before and after freeze drying, respectively. The prepared SLNs
had 71% loading efficiency. 90% of loaded curcumin was released after 48 hours.
Morphologic study for formulation was done by taking SEM pictures of curcumin SLNs.
Results show the spherical shape of curcumin SLNs. DSC studies were performed
to determine prolonged release mechanism. Antimicrobial studies were done to
compare the antimicrobial efficacy of curcumin SLNs with free curcumin. DSC
studies showed probability of formation of hydrogen bonds between cholesterol
and curcumin which resulted in prolonged release of curcumin. Lipid structure
of cholesterol could cause enhanced permeability in studied bacteria to
increase antibacterial characteristics of curcumin.
Conclusion: the designed curcumin SLNs could be candidate for
formulation of different dosage forms or cosmeceutical products.