Keyvan Yousefi
1, Sanaz Hamedeyazdan
2, Mohammadali Torbati
3, Fatemeh Fathiazad
2*1 Department of Pharmacology, Faculty of pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Department of Pharmacognosy, Faculty of pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Department of Traditional Pharmacy, Faculty of Traditional Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
Purpose: In the present study we aimed to quantify marrubiin,
as the major active compound, in the aerial parts of Marrubium vulgare
from Iran using a HPTLC-densitometry technique.
Methods: Quantitative determination of
marrubiin in M. vulgare methanol extract was performed by HPTLC
analysis via a fully automated TLC scanner. Later on, the in vitro antioxidant
activity of the M. vulgare methanol extract was determined using
1,1-diphenyl-2-picryl-hydrazil (DPPH) free radical scavenging assay.
Furthermore, total phenolics and flavonoids contents of the methanol extract
were quantified, spectrophotometrically.
Results: The amount of marrubiin
was calculated as 156 mg/g of M. vulgare extract. The antioxidant assay
revealed a strong radical scavenging activity for the M. vulgare
methanol extract with RC50 value of 8.24μg/mL. Total phenolics and
flavonoids contents for M. vulgare were determined as 60.4 mg gallic
acid equivalent and 12.05 mg quercetin equivalent per each gram of the extract,
correspondingly.
Conclusion: The presented
fingerprint of marrubiin in M. vulgare extract developed by HPTLC
densitometry afforded a detailed chemical profile, which might be useful in the
identification as well as quality evaluation of herbal medications based on M.
vulgare. Besides, the considerable antioxidant activity of M. vulgare
was associated with the presence of marrubiin along with phenolics and
flavonoids exerting a synergistic effect.