Evgenii Plotnikov
1*, Vladimir Silnikov
2, Andrew Gapeyev
3, Vladimir Plotnikov
41 Tomsk Polytechnic University, Tomsk, Russia.
2 Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia.
3 Institute of Cell Biophysics of Russian Acad. Sci., Pushchino, Russia.
4 Polytech Ltd, Tomsk, Russia.
Abstract
Purpose: The
problem of infectious diseases and drug resistance is becoming increasingly
important worldwide. Silver is extensively used as an anti-infective agent, but
it has significant toxic side effects. In this regard, it is topical to develop
new silver compounds with high biological activity and low toxicity. This work
is aimed to study DNA damage and chromosomal aberrations in blood cells under
the influence of new silver-based compound of general formula C6H19Ag2N4LiO6S2,
with antiviral activity.
Methods: The comet assay was applied for
the genotoxic affects assessment on mice blood leukocytes. DNA damage was
determined bases on the percentage of DNA in a comet tail (tail DNA), under the
influence of silver complex in different concentrations. Genotoxic effect of
the tested substance on the somatic cells was determined by chromosomal
aberration test of bone marrow cells of mice.
Results: In the course of the experiments,
no essential changes in the level of DNA damage in the cells were found, even
at highest concentrations. The administration of the substance in doses up to
2.5 g/kg in mice did not cause any increase in the frequency of chromosomal
aberration in bone marrow cells.
Conclusion:
Taking into account known silver
drug genotoxic properties, the use of a given complexed silver compound has
possible great advantages for potential applications in the treatment of
infectious diseases.