Hasan Bagherpour Shamloo
1,2, Saber Golkari
2, Zeinab Faghfoori
3,4, AliAkbar Movassaghpour
5, Hajie Lotfi
6, Abolfazl Barzegari
6, Ahmad Yari Khosroushahi
7,8*1 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Dryland Agricultural Research Institute (DARI), Agricultural Research, Education and Extension Organization (AREEO), Maragheh, Iran.
3 Tuberculosis & Lung Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Student Research Committee, Faculty of Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran.
5 Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
6 Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
7 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
8 Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
Purpose: Exposure to diazinon can
trigger acute and chronic toxicity and significantly induces DNA damage and
proapoptotic effects in different human cells. Due to the significance of
probiotic bacteria antitoxin effect, this study aimed to investigate the effect
of Lactobacillus casei on diazinon (DZN) cytotoxicity in human umbilical
vein endothelial cells (HUVEC) in vitro.
Methods: The cytotoxicity assessments
were performed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide) test, DAPI (4',6-diamidino-2-phenylindole) staining and flow
cytometric methodologies.
Results: Cytotoxic assessments through
flow cytometry/ DAPI staining demonstrated that apoptosis is the main cytotoxic
mechanism of diazinon in HUVEC cells and L. casei could decrease the
diazinon cytotoxic effects on toxicants.
Conclusion: the screen of total bacterial secreted
metabolites can be considered as a wealthy source to find the new active
compounds to introduce as reducing agricultural remained pesticide cytotoxicity
effects on the human food chain.