Adv Pharm Bull. 2016;6(4): 651-654.
doi: 10.15171/apb.2016.080
PMID: 28101473
PMCID: PMC5241424
Scopus ID: 85011357520
  Abstract View: 2304
  PDF Download: 1553

Short Communication

Purification and Characterization of Bovine Serum Albumin Using Chromatographic Method

Sanaz Balkani 1,2, Sara Shamekhi 1, Ramin Raoufinia 1, Reza Parvan 1, Jalal Abdolalizadeh 3,4*

1 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2 University of Tehran Kish International Campus, Tehran University, Tehran, Iran.
3 Paramedical faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
*Corresponding Author: Email: jabdolalizadeh@gmail.com


Purpose: Albumin is an abundant protein of blood and has many biopharmaceutical applications. The aim of this study was to purify bovine serum albumin (BSA) using produced rabbit anti-BSA antibody. Methods: The polyclonal antibody was produced against the BSA in rabbits. Then, the pure BSA was injected to three white New Zealand rabbits. ELISA test was done to evaluate antibody production. After antibody purification,the purified antibody was attached to CNBr-activated sepharose and finally it was used for purification of albumin from bovine serum. Western blotting analysis was used for functional assessment of immunoaffinity purified BSA. Results: The titer of anti-bovine albumin determined by ELISA was obtained 1: 256000. The SDS-PAGE showed up to 98% purity of isolated BSA and western blotting confirmed the BSA functionality. Purified bovine serum albumin by affinity chromatography showed a single band with molecular weight of 66 KDa. Conclusion: Affinity chromatography using produced rabbit anti-BSA antibody would be an economical and safe method for purification of BSA.
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Submitted: 26 Jun 2016
Revision: 19 Nov 2016
Accepted: 24 Nov 2016
ePublished: 22 Dec 2016
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