Logo-apb
Adv Pharm Bull. 2013;3(2): 367-371.
doi: 10.5681/apb.2013.059
PMID: 24312862
PMCID: PMC3848226
Scopus ID: 84885821153
  Abstract View: 1308
  PDF Download: 1102

Original Research

Thin Layer Chromatographic Analysis of Beta-Lactam Antibiotics

Gabriel Hancu*, Brigitta Simon, Hajnal Kelemen, Aura Rusu, Eleonora Mircia, Arpad Gyeresi
*Corresponding Author: Email: g_hancu@yahoo.com

Abstract

Purpose: The paper describes some thin layer chromatographic procedures that allow simple and rapid separation and identification of penicillins and cephalosporins from complex mixtures. Methods: Using silicagel GF254 as stationary phase and selecting different mobile phases we succeeded in the separation of the studied beta-lactamins. Our aim was not only to develop a simple, rapid and efficient method for their separation but also the optimization of the analytical conditions. Results: No system will separate all the beta-lactams, but they could be identified when supplementary information is used from color reactions and/or by using additional chromatographic systems. Conclusions: The right combination of solvent system and detection method allows the identification of the studied penicillins and cephalosporins and can be successfully used in the preliminary analysis beta-lactam antibiotics.
First Name
Last Name
Email Address
Comments
Security code


Abstract View: 1309

Your browser does not support the canvas element.


PDF Download: 1102

Your browser does not support the canvas element.

Submitted: 07 Feb 2013
Revision: 06 Apr 2013
ePublished: 20 Aug 2013
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)