Hajar Oghbaei
1, Naser Ahmadi Asl
1*, Farzam Sheikhzadeh
2, Mohammad Reza Alipour
1, Amir Mahdi khamaneh
31 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
3 Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
Purpose: The purpose of
this study was to investigate the regular moderate exercise effect on the
miR-192 expression changes in kidney of Streptozotocin- induced diabetic rats.
Methods: Forty adult male
Wistar rats were divided into four groups of 10, including Sedentary Control
group, Healthy 60 days Exercise group, diabetic group and Diabetic 60 days
Exercise. Diabetes was induced by injection of 60 mg/kg Streptozotocin and
after 48 hour blood glucose levels higher than 250 mg/dl were included to
diabetic rats. After 48 hour of induction diabetes, exercise protocol was
begun. Animals performed 5 days of consecutive treadmill exercise (60 min/day)
with 22 m/min speeds for 60 days. Kidney of the rats has removed and MicroRNA was extracted from kidney using miRCURYTM RNA
isolation kit.
Results: Exercise upregulated miR-192 expression
level significantly in the kidney of diabetic
rats in comparison to healthy group. There is not any significant change in
miR-192 expression in diabetic 60 days exercise compared to control group.
Conclusion: These results may indicate that exercise can help
to prevent the progression of diabetic nephropathy.