Adv Pharm Bull. 2019;9(1):70-75.
doi: 10.15171/apb.2019.009
  Abstract View: 227
  PDF Download: 214

Research Article

The Study of HLA-G Gene and Protein Expression in Patients withRecurrent Miscarriage

Elnaz Mosaferi 1 ORCiD, Nazila Alizadeh Gharamaleki 1 ORCiD, Laya Farzadi 2 ORCiD, Jafar Majidi 1 ORCiD, Zohreh Babaloo 1 ORCiD, Tohid kazemi 1, Mehrnoosh Ramezani 3 ORCiD, Meraj Tabatabaei 4 ORCiD, Hamid Ahmadi 5 ORCiD, Leili Aghebati maleki 1 ORCiD, Behzad Baradaran 1 * ORCiD

1 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Women Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Department of Biochemistry, school of Medicine, Ardabil University of Medical Science, Ardabil Iran.
4 Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5 Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.


Purpose: Although it has been frequently confirmed that HLA-G plays an important role in thereproduction and pregnancy, the pattern of HLA-G gene and its protein expression are rarelyaddressed in studies. Therefore we conducted this study in regard to evaluate the HLA-G geneand its protein expression in the women’s placenta with recurrent miscarriage.Methods: Placental samples were obtained from the women who were admitted for deliveryor abortion in Al Zahra and Taleghani hospitals, Tabriz, Iran. HLA-G gene expression wasdetermined by real-time polymerase chain reaction (PCR) and HLA-G protein expression wasassessed by western blotting and immunofluorescence staining in the tissue samples.Results: The results showed a significant decrease in the expression of gene and proteins ofHLA-G in the women with recurrent miscarriage compared to the control placental tissues.Conclusion: According to the obtained results, it was concluded that the decrement of HLA-Ggene and protein expressions are associated with recurrent miscarriage. Since there areconflicting results from other studies, it is suggested to conduct a more comprehensive similarstudy with greater sample size.
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Submitted: 19 Mar 2018
Revised: 26 Aug 2018
Accepted: 29 Sep 2018
First published online: 21 Feb 2019
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