Adv Pharm Bull. 2019;9(1):56-63.
doi: 10.15171/apb.2019.007
  Abstract View: 224
  PDF Download: 254

Research Article

The Effect of Mesenchymal Stem Cells on the Expression of IDOand Qa2 Molecules in Dendritic Cells

Ali Moravej 1 ORCiD, Amin Kouhpayeh 1 ORCiD, Bita Geramizadeh 2, Negar Azarpira 2 ORCiD, Ramin Yaghobi 2 ORCiD, Yaser Mansoori 1 ORCiD, Mohammad-Hossein Karimi 2 * ORCiD

1 Noncommunicable Diseases Research Centre, Fasa University of Medical Sciences, Fasa, Iran.
2 Transplant Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.

Abstract

Purpose: Mesenchymal stem cells (MSCs) have been shown to reduce the activity of immunecells, including dendritic cells (DCs). But the exact mechanism of mesenchymal inhibitionof DCs is still unknown. In this study, the effect of mesenchymal cells on the expression ofindoleamine dioxygenase (IDO) and Qa2 molecules in DCs was evaluated.Methods: MSCs and DCs were respectively isolated from the bone marrow and spleen of BALB/cmice. Then DCs were co-cultured with MSCs in the present and absence of lipopolysaccharides(LPS). Then the expression of mRNA and protein of IDO and Qa2 molecules were investigatedin DCs that were treated with MSCs.Results: The expression of IDO and Qa2 mRNA in DCs that were treated with MSCs did notsignificantly differ from the control group. The expression of IDO protein in DCs that were coculturedwith MSCs (in 1:10 and 1:50 ratios) in absence of LPS was increased, although theywere not statistically significant (P values: 0.24 and 0.18, respectively). The expression of Qa2protein in DCs that were co-cultured with MSCs (in 1:10 and 1:50 ratios) in presence of LPS wasincreased, although they were not statistically significant (P-values: 0.09 and 0.33, respectively).Conclusion: Our results denied the possibility that MSCs led to the induction of tolerogenic DCsby increasing the expression of the IDO and Qa2 immunomodulatory molecules.
Keywords: MSCs, DCs, IDO, Qa2, Immunomodulation
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Submitted: 02 Oct 2018
Revised: 10 Dec 2018
Accepted: 20 Dec 2018
First published online: 21 Feb 2019
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