Logo-apb
Adv Pharm Bull. 2021;11(2): 311-317.
doi: 10.34172/apb.2021.044
PMID: 33880353
PMCID: PMC8046404
  Abstract View: 1283
  PDF Download: 586
  Full Text View: 223

Research Article

Preparation, Physicochemical Characterization and Anti-Fungal Evaluation of Amphotericin B-Loaded PLGA-PEG-Galactosamine Nanoparticles

Ghobad Mohammadi 1* ORCID logo, Mostafa Fathian-Kolahkaj 2, Pardis Mohammadi 1 ORCID logo, Khosro Adibkia 3 ORCID logo, Ali Fattahi 1 ORCID logo

1 Pharmaceutical Sciences Research Center Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
2 Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
3 Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
*Corresponding Author: *Corresponding Author: Ghobad Mohammadi, Tel: +98 833 4302345, Fax: +98 833 4302345, Email: , Email: ghmohammadi@kums.ac.ir

Abstract

Purpose: The present study aimed to formulate PLGA and PLGA-PEG-galactosamine nanoparticles (NPs) loaded with amphotericin B with appropriate physicochemical properties and antifungal activity. PLGA was functionalized with GalN to increase the adhesion and antifungal activity of NPs against Candida albicans.

Methods: The physicochemical properties of NPs were characterized by particle size determination, zeta potential, drug crystallinity, loading efficiency, dissolution studies, differential scanning calorimeter (DSC), X-ray powder diffraction (XRPD), and Fourier transform infrared (FT-IR). Antifungal activity of the NPs at different drug/polymer ratios was examined by determining minimum inhibitory concentrations (MICs).

Results: the FT-IR and 1 HNMR analysis successfully confirmed the formation of PLGA- PEG-GalN NPs. The PLGA NPs were in the size range of 174.1 ± 3.49 to 238.2±7.59 nm while PLGA-GalN NPs were 255.6 ±4.08 nm in size, respectively. Loading efficiency was in the range of 67%±2.4 to 77%±1.6, and entrapment efficiency in the range of 68.185%±1.9 to 73.05%±0.6. Zeta potential and loading efficiency for PLGA-GalN NPs were –0.456, 71%. The NPs indicated an amorphous status according to XRPD patterns and DSC thermograms. The PLGA-PEG-GalN NPs showed higher fungistatic activity than PLGA NPs.

Conclusion: the results demonstrated that the antifungal activity of PLGA-PEG-GalN NPs was higher than pure amphotericin B and PLGA NPs.


First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 1284

Your browser does not support the canvas element.


PDF Download: 586

Your browser does not support the canvas element.


Full Text View: 223

Your browser does not support the canvas element.

Submitted: 14 May 2019
Revision: 11 Jul 2020
Accepted: 15 Jul 2020
ePublished: 15 Jul 2020
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)