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Adv Pharm Bull. 2020;10(1): 30-38.
doi: 10.15171/apb.2020.004
  Abstract View: 86
  PDF Download: 110

Research Article

Evaluating Inhibitory Effects of Paclitaxel and Vitamin D3 Loaded Poly Lactic Glycolic Acid Co-Delivery Nanoparticles on the Breast Cancer Cell Line

Sepideh Khodaverdi 1 ORCID logo, Alireza Jafari 2,3 * ORCID logo, Farahnaz Movahedzadeh 4,5, Fateme Madani 6, Arshid Yousefi Avarvand 7, Siavash Falahatkar 2

1 Department of Obstetrics and Gynecology, Fellowship of Laparoscopy, Endometriosis Research Center, Iran University of Medical Sciences, Tehran, Iran.
2 Urology Research Center, Razi Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
3 Cellular and Molecular Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
4 Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois, USA.
5 Department of Pharmaceutical Sciences, College of Pharmacy University of Illinois at Chicago, Chicago, Illinois, USA.
6 Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
7 Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Abstract

Purpose: Paclitaxel (PTX) has transpired as a significant agent in the treatment of breast cancer. Meanwhile, polylactic glycolic acid (PLGA) nanoparticles (NPs) are able to increase the anticancer effect of the PTX in the blood.

Methods: Nano-precipitation was used to prepare the PLGA-PTX-VitD3 co-delivery NPs. Drug loading, encapsulation efficiency, in vitro release profile, cell viability, migration, apoptosis, and bcl2 expression of NPs were evaluated.

Results: The average size of co-delivery NPs was 231 ± 46 nm. Observed was a controlled release of the PTX and vitamin D3 from co-delivery NPs between 0.5 and 240 hours. MTT showed the ability of 8 μg.mL-1 of co-delivery NPs to kill 50 % of the MCF-7; likewise, the co-delivery NPs prevented MCF-7 migration. The co-delivery NPs led 46.35 % MCF-7 to enter primary apoptosis. 60.8% of MCF-7 in the control group were able to enter the G (1) phase of the cell cycle. The co-delivery NPs increased expression of bax. In addition to its higher toxicity against MCF-7 than that of PTX, co-delivery NPs were able to release drugs continuously for a long period, which indeed increased the efficiency of the drugs.

Conclusion: The effect of co-delivery NPs on MCF-7 cell viability was different from that in other drugs. In fact, the co-deliver NPs were able to release drugs continuously for a long time, this could induce primary apoptosis in the MCF-7 and decrease the metastasis and toxicity of drugs.

Keywords: Breast cancer, Anzatax, 25-Hydroxyvitamin D2, PLGA compound, Nanoparticle, bax genes, MCF-7 cell
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Submitted: 22 May 2019
Revision: 23 Sep 2019
Accepted: 30 Sep 2019
ePublished: 11 Dec 2019
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