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Adv Pharm Bull. 2020;10(2): 254-263.
doi: 10.34172/apb.2020.030
  Abstract View: 86
  PDF Download: 93

Research Article

Characterization and Prospective Applications of the Exopolysaccharides Produced by Rhodosporidium babjevae

Rasool Mirzaei Seveiri 1 ORCID logo, Masoud Hamidi 2,3 ORCID logo, Cédric Delattre 4,5, Hamid Sedighian 1, Guillaume Pierre 4, Babak Rahmani 6, Sina Darzi 7, Clément Brasselet 4, Fatemeh Karimitabar 3, Ali Razaghpoor 8, Jafar Amani 1 * ORCID logo

1 Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
2 Department of Medical Biotechnology, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran.
3 Food and Drug Research Center, Vice-Chancellery of Food and Drug, Guilan University of Medical Sciences, Rasht, Iran.
4 Université Clermont Auvergne, CNRS, SIGMA Clermont, Institut Pascal, F-63000 Clermont-Ferrand, France.
5 Institut Universitaire de France (IUF), 1 rue Descartes, 75005 Paris, France
6 Department of Molecular Medicine, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
7 Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran.
8 Student Research Committee, Nursing and Midwifery Faculty, Qazvin University of Medical Sciences, Qazvin, Iran.
*Corresponding Author: Jafar Amani, Tel: +98 21 82482556, Fax: +98 21 88068924, Email: jafar.amani@gmail.com

Abstract

Purpose: Due to the potential industrial and therapeutic applications of the yeast exopolysaccharides (EPSs), there has been an increasing demand to assess these biopolymers with improved characteristics. This study aimed to characterize the EPSs from Rhodosporidium babjevae (ATCC 90942 and IBRC-M 30088) as well as to evaluate their possible antioxidant, emulsifying and antiproliferative activities.

Methods: Rhodosporidium babjevae was cultured for 5 days and following isolation of supernatant, EPSs precipitated with adding of cold absolute ethanol and freeze-dried. The EPSs chemical structure was determined by FT-IR, SEM, HPLC-SEC and GC-MS. Additionally the solubility, water holding capacity and emulsifying activity of EPSs were evaluated. In vitro, antioxidant activity was investigated against DPPH, superoxide and hydroxyl radicals. Finally the EPSs consequence on the cell proliferation of human breast adenocarcinoma (MCF-7) and Madin-Darby canine kidney (MDCK) cell lines was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test.

Results: R. babjevae excreted 1.6±0.2 g/L of the EPSs. The EPSs had three fractions with molecular weights of 1.02 ×106 , 5×105 and 2×105 Da. Mannose and glucose were found as the main monosaccharides of the EPSs (84:16 mol%, respectively). The EPSs exhibited emulsifying activity on sun flower oil. The scavenging activities were found to be dose-dependent and higher than hyaluronic acid. Significant difference among the EPSs treatments on the proliferation of MCF-7 and MDCK cell lines was not observed (P>0.05).

Conclusion: These results show the interesting potential of the EPSs from R. babjevae as biocompatible compounds for using in food and pharmaceutical fields.

Keywords: Exopolysaccharides, Rhodosporidium babjevae, Antioxidant activity, Emulsifying activity, Antiproliferative activity
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Submitted: 04 Jun 2019
Revision: 27 Nov 2019
Accepted: 08 Dec 2019
ePublished: 18 Feb 2020
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