Logo-apb
Adv Pharm Bull. 2020;10(2): 233-238.
doi: 10.34172/apb.2020.027
PMID: 32373491
PMCID: PMC7191233
Scopus ID: 85088699264
  Abstract View: 350
  PDF Download: 304

Research Article

Solubilization of Human Interferon β-1b Inclusion Body Proteins by Organic Solvents

Samira Nekoufar 1 ORCID logo, Ahmad Fazeli 2 ORCID logo, Mohammad Reza Fazeli 3* ORCID logo

1 Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
2 The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences (TUMS), Tehran, Iran.
3 Department of Drug & Food Control, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran.
*Corresponding Author: Mohammad Reza Fazeli, Fax: +98 21 6959060, Email: fazelimo@sina.tums.ac.ir

Abstract

Purposes: Solubilization of inclusion bodies expressed in E. coli is a critical step during manufacturing of recombinant proteins expressed as inclusion bodies. So far, various methods have been used for solubilization and purification of inclusion body proteins to obtain active proteins with high purity and yield. The aim of this study was to examine the benefit of organic solvents such as alcohols in solubilization of recombinant interferon β-1b inclusion bodies.

Methods: Effect of important parameters inclusion pH, concentration and type of denaturant and concentration of alcoholic solvents were optimized to formulate a suitable solubilization buffer and investigate their effect on solubilization of interferon β-1b inclusion bodies.

Results: Our findings showed the acidic pH in the range of 2-3 is more suitable than alkaline pH >12 for solubilization and achieving higher content of interferon β-1beta and pure recombinant protein. We have also demonstrated that 1% SDS acts better than 2M urea to solubilize Inclusion body proteins of interferon β-1b at pH of 2-3. The interferon concentration was 2.35 mg per 100 mg IB when we used 40% (v/v) 1-propanol and 20% (v/v) 2-butanol into the buffer solution as well.

Conclusion: The optimized method provides gentile condition for solubilization of inclusion body at high protein concentration and purity with a degree of retention of native secondary structure which makes this method valuable to be used in production and research area.

Keywords: Interferon beta-1b, Inclusion body, Solubilization, Organic solvent, Alcohol
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 350

Your browser does not support the canvas element.


PDF Download: 304

Your browser does not support the canvas element.

Submitted: 09 Jun 2019
Revision: 07 Sep 2019
Accepted: 10 Oct 2019
ePublished: 18 Feb 2020
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)