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Adv Pharm Bull. 2020;10(3): 408-417.
doi: 10.34172/apb.2020.049
  Abstract View: 59
  PDF Download: 69

Research Article

Formulation and in Vitro Evaluation of Casein Nanoparticles as Carrier for Celecoxib

Jyotsana R. Madan 1 * ORCID logo, Izharahemad N. Ansari 1, Kamal Dua 2 ORCID logo, Rajendra Awasthi 3 ORCID logo

1 Department of Pharmaceutics, Smt. Kashibai Navale College of Pharmacy, Savitribai Phule Pune University, Pune 411048, Maharashtra, India.
2 Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Ultimo NSW 2007, Australia.
3 Amity Institute of Pharmacy, Amity University Uttar Pradesh, Noida 201313, India.

Abstract

Purpose: The objective of this work was to formulate casein (CAS) nanocarriers for the dissolutionenhancement of poorly water soluble drug celecoxib (CLXB).Methods: The CLXB loaded CAS nanocarriers viz., nanoparticles, reassembled CAS micellesand nanocapsules were prepared using sodium caseinate (SOD-CAS) as a carrier to enhance thesolubility of CLXB. The prepared formulations were characterized for particle size, polydispersityindex, zeta potential, percentage entrapment efficiency, and surface morphology for the selectionof best formulation. Fourier transform infrared spectroscopy, differential scanning calorimetryand X-ray powder diffraction study was used to for the confirmation of encapsulation of CLXB.Further, in vitro drug dissolution, ex-vivo permeation studies on chicken ileum and stabilitystudies were carried out.Results: The CLXB loaded casein nanoparticles (CNP) (batch A2) showed a particle size diameter216.1 nm, polydispersity index 0.422 with percentage entrapment efficiency of 90.71% andzeta potential of -24.6 mV. Scanning electron microscopy of suspension confirmed globularshape of CNP. The in vitro release data of optimized batch followed non Fickian diffusionmechanism. The ex vivo permeation studies on chicken ileum of CLXB loaded CNP showedpermeation through mucous membrane as compared to pure CLXB. The apparent permeabilityof best selected freeze dried CLXB loaded CNP (batch A2) was higher and gradually increasedfrom 0.90 mg/cm2 after 10 min to a maximum of 1.95 mg/cm2 over the subsequent 90 min. Ahigher permeation was recorded at each time point than that of the pure CLXB.Conclusion: The study explored the potential of CAS as a carrier for solubility enhancement ofpoorly water soluble drugs.
Keywords: Casein nanoparticles, Celecoxib, Nanocarrier, Reassembled casein micelles, Sodium caseinate
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Submitted: 28 Jul 2019
Revision: 28 Jan 2020
Accepted: 03 Feb 2020
ePublished: 11 May 2020
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